Niilo L, Macdonald D W, Godkin G F, Stone M W
Can Vet J. 1986 Jun;27(6):245-9.
Two parallel surveys of rams from Alberta sheep flocks were conducted to determine the presence of infection with Brucella ovis. In a retrospective study over a period of 24 months, using complement fixation test, 12 flocks out of 142 tested were considered infected. In another 17-month survey of slaughter rams by serology and culture methods 11 flocks out of 124 were found to be infected. The overall prevalence of ovine brucellosis was 8.6% of the flocks tested which represented 12.5% of the estimated sheep flocks in Alberta. Up to 67% of rams in infected flocks reacted to complement fixation test.The complement fixation test was evaluated for its efficiency in the diagnosis of ovine brucellosis and compared with a limited number of an enzyme-linked immunosorbent assay (ELISA) results and clinical criteria. The complement fixation test as well as ELISA identified all culture positive rams. Both serological tests appeared satisfactory for the diagnosis of B. ovis epididymitis when the results could be interpreted in the light of flock history and clinical findings.
对来自艾伯塔省羊群的公羊进行了两项平行调查，以确定是否存在绵羊布鲁氏菌感染。在一项为期24个月的回顾性研究中，采用补体结合试验，142个受试羊群中有12个被认为受到感染。在另一项为期17个月的对公羊屠宰样本进行血清学和培养方法的调查中，124个羊群中有11个被发现受到感染。绵羊布鲁氏菌病的总体患病率为受试羊群的8.6%，占艾伯塔省估计羊群数量的12.5%。在受感染的羊群中，高达67%的公羊对补体结合试验呈阳性反应。对补体结合试验在绵羊布鲁氏菌病诊断中的效率进行了评估，并与有限数量的酶联免疫吸附测定（ELISA）结果及临床标准进行了比较。补体结合试验和ELISA都能识别所有培养阳性的公羊。当根据羊群病史和临床发现来解释结果时，这两种血清学检测方法对于绵羊附睾布鲁氏菌病似乎都令人满意。
