Altayb Hisham N, Chaieb Kamel, Baothman Othman, Alzahrani Faisal A, Zamzami Mazin A, Almugadam Babiker Saad
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Center of Artificial Intelligence in Precision Medicines, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Saudi J Biol Sci. 2022 Jul;29(7):103317. doi: 10.1016/j.sjbs.2022.103317. Epub 2022 May 23.
The diversity of oral microbiota is affected by diets habits, gender, age, ethnic group, and environment. The acquisition of oral microbiota and the role of family on oral microbiota development is poorly understood. This study aims to characterize and compare the oral bacterial microbiota among families using 16S rRNA gene sequencing. This work was conducted in Jeddah city from 2020 to 2021, in which four families composed of 20 members of different ethnicity and lifestyle were recruited. After the collection of saliva samples, the DNA was extracted and processed for 16S rRNA gene metagenomics sequencing. Among 378 OUTs generated, 39 (10.3%) were unique in group A, 13 (3.4%) unique in group B, and 11 (2.9%) were unique in groups C and D. We observed a significant variation at the level of top abundance phylum (14), families (23), genera (24), and species (22) of bacteria among family members. Within family groups, different bacterial species were reported to be more dominant among certain family members than the other; and and were more dominant in parents of some families than the other family member. In summary, this study highlights the precise and perceptible association of oral microbial between family members. Our findings documented the clustering of certain bacterial species in family groups, supporting the role of community in the development of oral microbiota.
口腔微生物群的多样性受饮食习惯、性别、年龄、种族和环境的影响。人们对口腔微生物群的获得以及家庭在口腔微生物群发育中的作用了解甚少。本研究旨在利用16S rRNA基因测序对家庭中的口腔细菌微生物群进行表征和比较。这项工作于2020年至2021年在吉达市进行，招募了四个家庭，共20名成员，他们具有不同的种族和生活方式。收集唾液样本后，提取DNA并进行16S rRNA基因宏基因组测序。在产生的378个操作分类单元中，39个（10.3%）在A组中是独特的，13个（3.4%）在B组中是独特的，11个（2.9%）在C组和D组中是独特的。我们观察到家庭成员之间在细菌丰度最高的门（14个）、科（23个）、属（24个）和种（22个）水平上存在显著差异。在家庭组中，据报道某些家庭成员中不同的细菌种类比其他成员更占优势；在一些家庭的父母中比其他家庭成员更占优势。总之，本研究强调了家庭成员之间口腔微生物的精确且可感知的关联。我们的研究结果记录了家庭组中某些细菌种类的聚集，支持了群落在口腔微生物群发育中的作用。
