Solé-Martí Xavi, Vilella Tània, Labay Cédric, Tampieri Francesco, Ginebra Maria-Pau, Canal Cristina
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, and Research Centre for Biomedical Engineering (CREB), Universitat Politècnica de Catalunya (UPC), c/Eduard Maristany 14, 08019 Barcelona, Spain.
Barcelona Research Center in Multiscale Science and Engineering, UPC, 08019 Barcelona, Spain.
Biomater Sci. 2022 Jul 12;10(14):3845-3855. doi: 10.1039/d2bm00308b.
Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies. Acting as delivery vehicles that allow a controlled release of RONS to the diseased site, plasma-treated hydrogels can overcome some of the limitations presented by plasma-treated liquids in therapies. In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS. MC hydrogels demonstrated the capacity for generation, prolonged storage and release of RONS. This release induced cytotoxic effects on the osteosarcoma cancer cell line MG-63, reducing its cell viability in a dose-response manner. These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.
水凝胶最近被提议作为在气体等离子体处理后产生活性氧和氮物种（RONS）的合适材料，并被假定为传统癌症治疗的有前途的替代方案。作为允许RONS可控释放到患病部位的递送载体，经等离子体处理的水凝胶可以克服等离子体处理液体在治疗中存在的一些局限性。在这项工作中，我们优化了甲基纤维素（MC）水凝胶的组成，使其能够在生理温度下形成凝胶，同时在室温下保持液相，以便进行气体等离子体处理并适当形成等离子体产生的RONS。MC水凝胶展示了产生、长时间储存和释放RONS的能力。这种释放对骨肉瘤癌细胞系MG-63产生了细胞毒性作用，以剂量反应方式降低了其细胞活力。这些有前景的结果表明，经等离子体处理的热敏水凝胶是提供局部抗癌治疗的良好候选材料。
