Amberson J B, Vaughan E D, Gray G F, Naus G J
Cancer. 1987 Jun 15;59(12):2091-5. doi: 10.1002/1097-0142(19870615)59:12<2091::aid-cncr2820591221>3.0.co;2-u.
Nuclear DNA content of paraffin-embedded tissue from 48 adrenocortical neoplasms, 18 histologically normal control adrenal glands, and five hyperplastic adrenal glands was analyzed retrospectively using flow cytometry. Aneuploidy was compared with morphologic criteria as a predictor of recurrence. All 18 controls, five hyperplastic glands, and 39 neoplasms were diploid. Nine neoplasms were aneuploid. Compared with their diploid counterparts, aneuploid neoplasms were more likely to weigh more than 50 g (P less than 0.0001) and to have three or more histologic features of carcinoma (P less than 0.0001). Thirty-six neoplasms were followed clinically for at least 2 years (range 24 to 120 months, mean = 64.6 months) or until local recurrence, metastasis, or death. Five were clinically malignant. Neoplasms which recurred or metastasized were more apt to be aneuploid (P less than 0.005) than those showing no evidence of further disease during the follow-up period. They were also more likely to weigh more than 50 g (P less than 0.005) and to have three or more histologic features of carcinoma (P less than 0.0025). However, neither aneuploidy, large size, nor unfavorable histology result was a consistent feature in every malignant neoplasm. Flow cytometric DNA content analysis appears to be as effective a predictor of clinical outcome as size and histology and may be of particular value when the morphologic features are ambiguous.
采用流式细胞术对48例肾上腺皮质肿瘤、18例组织学正常的对照肾上腺及5例增生性肾上腺的石蜡包埋组织的核DNA含量进行回顾性分析。将非整倍体与形态学标准进行比较，作为复发的预测指标。所有18例对照、5例增生性腺及39例肿瘤均为二倍体。9例肿瘤为非整倍体。与二倍体肿瘤相比，非整倍体肿瘤更可能重量超过50g（P<0.0001）且具有三种或更多癌的组织学特征（P<0.0001）。36例肿瘤进行了至少2年（范围24至120个月，平均=64.6个月）的临床随访，直至局部复发、转移或死亡。5例为临床恶性肿瘤。复发或转移的肿瘤比随访期间无进一步疾病证据的肿瘤更易为非整倍体（P<0.005）。它们也更可能重量超过50g（P<0.005）且具有三种或更多癌的组织学特征（P<0.0025）。然而，非整倍体、大尺寸或不良组织学结果并非每个恶性肿瘤的一致特征。流式细胞术DNA含量分析似乎与大小和组织学一样是临床结果的有效预测指标，当形态学特征不明确时可能具有特殊价值。
