Bowlby L S, DeBault L E, Abraham S R
Cancer. 1986 Oct 1;58(7):1499-505. doi: 10.1002/1097-0142(19861001)58:7<1499::aid-cncr2820580721>3.0.co;2-v.
Nuclear DNA contents of 22 paraffin-embedded adrenal cortical tumors (16 adenomas and 6 carcinomas) were determined by flow cytometric analysis. While all of the sixteen adenomas had a diploid pattern, 83.3% (5/6) of the carcinomas were aneuploid. Three of the six patients with carcinomas (50%) developed metastases and died of their disease; all had aneuploid tumors. The three living patients with carcinoma included two children, with aneuploid tumors, and one adult, with a diploid tumor. Large tumor size correlated with aneuploidy. This study indicated that flow cytometric analysis of nuclear DNA content complements conventional histopathologic methods in the diagnosis of malignancy in adrenal cortical tumors. It may also prove to be a valuable tool in predicting the prognosis of patients with adrenal cortical carcinomas.
通过流式细胞术分析确定了22例石蜡包埋的肾上腺皮质肿瘤（16例腺瘤和6例癌）的核DNA含量。16例腺瘤均为二倍体模式，而83.3%（5/6）的癌为非整倍体。6例癌患者中有3例（50%）发生转移并死于该病；所有患者均有非整倍体肿瘤。3例存活的癌患者包括2名儿童，肿瘤为非整倍体，1名成人，肿瘤为二倍体。肿瘤体积大与非整倍体相关。本研究表明，核DNA含量的流式细胞术分析可补充传统组织病理学方法用于肾上腺皮质肿瘤恶性肿瘤的诊断。它也可能被证明是预测肾上腺皮质癌患者预后的有价值工具。
