Key Laboratory of Prevention and Control Agents for Animal Bacteriosis, Ministry of Agriculture and Rural Affairs; Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, 430064, PR China; Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, 430064, Hubei, PR China.
College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, PR China.
Microb Pathog. 2021 Sep;158:105118. doi: 10.1016/j.micpath.2021.105118. Epub 2021 Jul 30.
Porcine circovirus type 2 (PCV2) can cause various clinical diseases in pigs, resulting in huge losses for the pig farms all over the world. In order to develop a new strategy to control PCV2, it is essential to understand its mechanisms firstly, especially PCV2 interferes with the host's innate immunity. In the present study, lncRNA and mRNA expression profiles in porcine lymphnode response to PCV2 infection were deeply sequenced and analyzed. 3271 novel lncRNAs were identified in all. 1898 mRNAs and 282 lncRNAs showed differential expression between control and PCV2-infected groups. The bioinformatics analysis including lncRNA-mRNA co-expression network construction, as well as GO and KEGG pathway analysis focused on the DEGs was carried out. The results indicated that lncRNAs might participate in PCV2 infection-induced the pathogenesis of immunosuppression through regulating the host's immune responses, biological regulation, response to stimulus, cellular component organization or biogenesis and metabolism. And these differentially expressed lncRNAs might play important roles in response to PCV2 infection in the host's innate immune system. These findings provided a large-scale survey of dysregulated lncRNAs after PCV2 infection, especially the lncRNAs responded to host's innate immune within the lymphnode. This study will provide a novel insight into the lncRNAs' functions and the possible immunosuppressive mechanism induced by PCV2 infection. However, further research will be required to verify the characteristic function of the dysregulated lncRNAs.
猪圆环病毒 2 型（PCV2）可引起猪的多种临床疾病，给世界各地的养猪场造成巨大损失。为了开发控制 PCV2 的新策略，首先必须了解其机制，特别是 PCV2 干扰宿主的固有免疫。本研究通过深度测序和分析，研究了猪淋巴结对 PCV2 感染的 lncRNA 和 mRNA 表达谱。在所有样本中鉴定出 3271 个新的 lncRNA。对照组和 PCV2 感染组之间有 1898 个 mRNAs 和 282 个 lncRNAs 表现出差异表达。包括 lncRNA-mRNA 共表达网络构建以及 GO 和 KEGG 通路分析的生物信息学分析集中在 DEGs 上。结果表明，lncRNA 可能通过调节宿主的免疫反应、生物调节、对刺激的反应、细胞成分组织或生物发生和代谢，参与 PCV2 感染诱导的免疫抑制发病机制。这些差异表达的 lncRNA 可能在宿主固有免疫系统对 PCV2 感染的反应中发挥重要作用。这些发现为 PCV2 感染后失调的 lncRNA 提供了大规模调查，特别是在淋巴结中对宿主固有免疫的反应。该研究将为 lncRNA 的功能和 PCV2 感染诱导的可能免疫抑制机制提供新的见解。然而，需要进一步的研究来验证失调的 lncRNA 的特征功能。
