Kuang Weiyang, Yang Jiajia, Liu Zhiyuan, Zeng Jinzi, Xia Xuewei, Chen Xiaodan, Zhong Saiyi, Huang Riming
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
Foods. 2022 May 27;11(11):1572. doi: 10.3390/foods11111572.
Catechin possesses a potential anti-inflammatory activity, but its anti-inflammatory mechanism is still unclear. Herein, the analysis of network pharmacology showed that catechin might mediate ferroptosis on macrophages to exhibit a significant anti-inflammatory effect on RAW264.7. The metabolomics further indicated that catechin might influence ferroptosis by activating two pathways of cysteine and methionine metabolism and glutathione metabolism, and inhibiting the pathway of ferroptosis to promote the reduction of l-methionine-s-oxide and s-glutathionyl-l-cysteine, and the reduction and synthesis of γ-glutamylcysteine. Furthermore, related proteins (MSRA, CDR, GSR and GCL) in three metabolic pathways and ferroptosis-related proteins (GPX4 and SLC7A11) might be relevant to catechin through molecular docking. Thus, we speculate that catechin plays an anti-inflammatory effect through mediating ferroptosis on RAW264.7, which still needs further focus on the detailed molecular mechanism.
儿茶素具有潜在的抗炎活性，但其抗炎机制仍不清楚。在此，网络药理学分析表明，儿茶素可能通过介导巨噬细胞铁死亡，从而对RAW264.7细胞发挥显著的抗炎作用。代谢组学进一步表明，儿茶素可能通过激活半胱氨酸和蛋氨酸代谢以及谷胱甘肽代谢这两条途径来影响铁死亡，并抑制铁死亡途径，从而促进L-蛋氨酸-S-氧化物和S-谷胱甘肽基-L-半胱氨酸的减少，以及γ-谷氨酰半胱氨酸的减少和合成。此外，通过分子对接发现，三条代谢途径中的相关蛋白（MSRA、CDR、GSR和GCL）以及铁死亡相关蛋白（GPX4和SLC7A11）可能与儿茶素相关。因此，我们推测儿茶素通过介导RAW264.7细胞的铁死亡发挥抗炎作用，但其详细分子机制仍需进一步关注。
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