Ji Da, Su Xin, Yao Junjie, Zhang Wenzheng, Wang Rongrong, Zhang Shuhai
Research Center of Fishery Resources and Environment, Guizhou University, Guiyang 550000, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guiyang 550000, China.
Animals (Basel). 2022 May 27;12(11):1377. doi: 10.3390/ani12111377.
The aim of this study was to assess the current status of the germplasm resources of golden-backed carp ( var. ) cultured in paddy fields in Guizhou Province, China. Five populations of golden-backed carp in Liping County, Jinping County, Huangping County, Congjiang County and Duyun City in Guizhou Province were subjected to high-throughput sequencing by 2b-RAD technology, and their genetic diversity and genetic differentiation were analysed. Based on sequencing, 44,896 SNP loci were obtained, and all five population genetic diversity indicators showed low diversity. In the NJ tree, the Congjiang and Liping populations were mixed together, and the other three groups formed a cluster. A cross-validation error box plot and pong cluster plot were constructed to show the K value results. When K = 1, the cross-validation error rate was the lowest. Principal component analysis showed that the Duyun population formed a group separate from the group comprising the other four populations. The genetic differentiation index and genetic distances between the Duyun population and the remaining four populations were greater than 0.05, indicating population differentiation. The genetic diversity of the five populations of golden-backed carp in Guizhou Province was low, the genetic differentiation of the Duyun population was the most significant, and the Duyun population was separate from the other four groups.
本研究旨在评估中国贵州省稻田养殖的荷包鲤（变种）种质资源现状。对贵州省黎平县、锦屏县、黄平县、从江县和都匀市的5个荷包鲤群体采用2b-RAD技术进行高通量测序，并分析其遗传多样性和遗传分化。基于测序获得了44896个SNP位点，5个群体的遗传多样性指标均显示多样性较低。在NJ树中，从江群体和黎平群体混合在一起，其他3个群体聚为一支。构建交叉验证误差箱线图和pong聚类图展示K值结果，当K = 1时，交叉验证错误率最低。主成分分析显示，都匀群体单独聚为一支，与其他4个群体组成的一支分开。都匀群体与其余4个群体间的遗传分化指数和遗传距离均大于0.05，表明群体间存在分化。贵州省5个荷包鲤群体的遗传多样性较低，都匀群体的遗传分化最为显著，且都匀群体与其他4个群体分开。
