Jin Peng, Gao Shiqi, He Long, Xu Miaoze, Zhang Tianye, Zhang Fan, Jiang Yaoyao, Liu Tingting, Yang Jin, Yang Jian, Dai Liangying, Chen Jianping
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.
State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Plants (Basel). 2020 Dec 24;10(1):19. doi: 10.3390/plants10010019.
Histone acetylation is a dynamic modification process co-regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Although HDACs play vital roles in abiotic or biotic stress responses, their members in and their response to plant viruses remain unknown. Here, we identified and characterized 49 at the whole-genome level. Based on phylogenetic analyses, could be divided into 5 clades, and their protein spatial structure was integral and conserved. Chromosomal location and synteny analyses showed that were widely distributed on wheat chromosomes, and gene duplication has accelerated the gene family evolution. The cis-acting element analysis indicated that were involved in hormone response, light response, abiotic stress, growth, and development. Heatmaps analysis of RNA-sequencing data showed that genes were involved in biotic or abiotic stress response. Selected were differentially expressed in diverse tissues or under varying temperature conditions. All selected were significantly upregulated following infection with the (BSMV), (CWMV), and (WYMV), suggesting their involvement in response to viral infections. Furthermore, -silenced contributed to increasing wheat resistance against CWMV infection. In summary, these findings could help deepen the understanding of the structure and characteristics of the gene family in wheat and lay the foundation for exploring the function of in plants resistant to viral infections.
组蛋白乙酰化是一个由组蛋白乙酰转移酶（HATs）和组蛋白去乙酰化酶（HDACs）共同调控的动态修饰过程。尽管HDACs在非生物或生物胁迫应答中发挥着重要作用，但其在小麦中的成员及其对植物病毒的应答仍不清楚。在此，我们在全基因组水平上鉴定并表征了49个HDACs。基于系统发育分析，HDACs可分为5个进化枝，其蛋白质空间结构完整且保守。染色体定位和共线性分析表明，HDACs广泛分布于小麦染色体上，基因复制加速了HDAC基因家族的进化。顺式作用元件分析表明，HDACs参与激素应答、光应答、非生物胁迫、生长和发育。RNA测序数据的热图分析表明，HDAC基因参与生物或非生物胁迫应答。所选的HDACs在不同组织或不同温度条件下差异表达。所有所选的HDACs在感染大麦条纹花叶病毒（BSMV）、中国小麦花叶病毒（CWMV）和小麦黄花叶病毒（WYMV）后均显著上调，表明它们参与了对病毒感染的应答。此外，HDAC基因沉默有助于提高小麦对CWMV感染的抗性。总之，这些发现有助于加深对小麦HDAC基因家族结构和特征的理解，并为探索HDACs在植物抗病毒感染中的功能奠定基础。
