E3 ligases promote protein ubiquitination and degradation, which regulate every aspect of eukaryotic life. The Ariadne (ARI) proteins of RBR (ring between ring fingers) protein subfamily has been discovered as a group of potential E3 ubiquitin ligases. Only a few available research studies show their role in plant adaptations processes against the external environment. Presently, the functions of ARI proteins are largely unknown in plants. Therefore, in this study, we performed genome-wide analysis to identify the gene family and explore their potential importance in . A total of 39 genes were identified in the genome and were classified into three subfamilies (A, B and C) based on phylogenetic analysis. The protein-protein interaction networks and enrichment analysis indicated that genes could be involved in endoreduplication, DNA repair, proteasome assembly, ubiquitination, protein kinase activity and stress adaptation. The transcriptome data analysis in various tissues provided us an indication of some genes' functional importance in tissue development. We also identified potential genes that were significantly responsive towards the abiotic stresses. Furthermore, eight genes were identified as candidate genes for multiple agronomic traits through association mapping analysis in ; among them, , which is the ortholog of , was significantly associated with ten agronomic traits. This study provided useful information on genes, which could aid targeted functional research and genetic improvement for breeding in .