• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过. 比较转录组分析鉴定花特异启动子

Identification of Flower-Specific Promoters through Comparative Transcriptome Analysis in .

机构信息

Key Laboratory of Biology and Genetics Improvement of Oil Crops, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China.

出版信息

Int J Mol Sci. 2019 Nov 26;20(23):5949. doi: 10.3390/ijms20235949.

DOI:10.3390/ijms20235949
PMID:31779216
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6928827/
Abstract

(oilseed rape) is an economically important oil crop worldwide. Sclerotinia stem rot (SSR) caused by is a threat to oilseed rape production. Because the flower petals play pivotal roles in the SSR disease cycle, it is useful to express the resistance-related genes specifically in flowers to hinder further infection with . To screen flower-specific promoters, we first analyzed the transcriptome data from 12 different tissues of the line ZS11. In total, 249 flower-specific candidate genes with high expression in petals were identified, and the expression patterns of 30 candidate genes were verified by quantitative real-time transcription-PCR (qRT-PCR) analysis. Furthermore, two novel flower-specific promoters ( and promoter) were identified, and the tissue specificity and continuous expression in petals were determined in transgenic with fusing the promoters to -glucuronidase ()-reporter gene. GUS staining, transcript expression pattern, and GUS activity analysis indicated that and promoter were strictly flower-specific promoters, and promoter had a stronger activity. The two promoters were further confirmed to be able to direct expression in flowers using transient expression system. The transcriptome data and the flower-specific promoters screened in the present study will benefit fundamental research for improving the agronomic traits as well as disease and pest control in a tissue-specific manner.

摘要

(油菜)是一种在全球范围内具有重要经济意义的油料作物。由 引起的菌核病(SSR)对油菜生产构成威胁。由于花瓣在 SSR 疾病循环中起着关键作用，因此特异性表达与抗性相关的基因以阻碍与 的进一步感染是很有用的。为了筛选出花特异性启动子，我们首先分析了 品系 ZS11 的 12 种不同组织的转录组数据。总共鉴定出 249 个在花瓣中高表达的花特异性候选基因，并通过定量实时转录-PCR(qRT-PCR)分析验证了 30 个候选基因的表达模式。此外，还鉴定出了两个新的花特异性启动子( 和 启动子)，并通过将启动子与β-葡萄糖醛酸酶(-glucuronidase，GUS)-报告基因融合，在转基因 中确定了其在花瓣中的组织特异性和连续表达。GUS 染色、转录表达模式和 GUS 活性分析表明， 和 启动子是严格的花特异性启动子， 启动子活性更强。使用瞬时表达系统进一步证实这两个启动子能够在 花朵中指导 表达。本研究中筛选出的转录组数据和花特异性启动子将有助于基础研究，以改善油菜的农艺性状，并以组织特异性的方式控制疾病和害虫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/bdb3c5d41ec3/ijms-20-05949-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/885681a27f94/ijms-20-05949-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/bf1fe20cdf06/ijms-20-05949-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/07142c9342f7/ijms-20-05949-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/850a8a8cc908/ijms-20-05949-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/9ecdca1668e8/ijms-20-05949-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/bdb3c5d41ec3/ijms-20-05949-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/885681a27f94/ijms-20-05949-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/bf1fe20cdf06/ijms-20-05949-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/07142c9342f7/ijms-20-05949-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/850a8a8cc908/ijms-20-05949-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/9ecdca1668e8/ijms-20-05949-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a985/6928827/bdb3c5d41ec3/ijms-20-05949-g006.jpg

相似文献

1
Identification of Flower-Specific Promoters through Comparative Transcriptome Analysis in .通过. 比较转录组分析鉴定花特异启动子
Int J Mol Sci. 2019 Nov 26;20(23):5949. doi: 10.3390/ijms20235949.
2
Production of red-flowered oilseed rape via the ectopic expression of Orychophragmus violaceus OvPAP2.通过异位表达 Orychophragmus violaceus OvPAP2 生产红花油菜。
Plant Biotechnol J. 2018 Feb;16(2):367-380. doi: 10.1111/pbi.12777. Epub 2017 Jul 26.
3
Transforming petals into sepaloid organs in Arabidopsis and oilseed rape: implementation of the hairpin RNA-mediated gene silencing technology in an organ-specific manner.在拟南芥和油菜中将花瓣转化为萼状器官：以器官特异性方式实施发夹RNA介导的基因沉默技术。
Planta. 2004 Jan;218(3):379-87. doi: 10.1007/s00425-003-1117-1. Epub 2003 Oct 8.
4
Molecular analysis and expression of a floral organ-specific polygalacturonase gene isolated from rapeseed (Brassica napus L.).从油菜（甘蓝型油菜）中分离的花器官特异性多聚半乳糖醛酸酶基因的分子分析与表达。
Mol Biol Rep. 2010 Dec;37(8):3851-62. doi: 10.1007/s11033-010-0041-2. Epub 2010 Mar 7.
5
Interactions of WRKY15 and WRKY33 transcription factors and their roles in the resistance of oilseed rape to Sclerotinia infection.WRKY15 和 WRKY33 转录因子的相互作用及其在油菜对菌核病抗性中的作用。
Plant Biotechnol J. 2018 Apr;16(4):911-925. doi: 10.1111/pbi.12838. Epub 2017 Nov 9.
6
Cell specific, cross-species expression of myrosinases in Brassica napus, Arabidopsis thaliana and Nicotiana tabacum.黑芥子酶在甘蓝型油菜、拟南芥和烟草中的细胞特异性跨物种表达。
Plant Mol Biol. 2004 Mar;54(4):597-611. doi: 10.1023/B:PLAN.0000038272.99590.10.
7
Genome-wide identification of the NPR1-like gene family in Brassica napus and functional characterization of BnaNPR1 in resistance to Sclerotinia sclerotiorum.甘蓝型油菜 NPR1 样基因家族的全基因组鉴定及 BnaNPR1 基因在抗核盘菌中的功能分析。
Plant Cell Rep. 2020 Jun;39(6):709-722. doi: 10.1007/s00299-020-02525-z. Epub 2020 Mar 5.
8
Comparative transcriptomic analysis uncovers the complex genetic network for resistance to Sclerotinia sclerotiorum in Brassica napus.比较转录组分析揭示了甘蓝型油菜对核盘菌抗性的复杂遗传网络。
Sci Rep. 2016 Jan 8;6:19007. doi: 10.1038/srep19007.
9
The Sclerotinia sclerotiorum-inducible promoter pBnGH17D7 in Brassica napus: isolation, characterization, and application in host-induced gene silencing.甘蓝型油菜中受核盘菌诱导的启动子 pBnGH17D7 的分离、鉴定及在寄主诱导基因沉默中的应用。
J Exp Bot. 2022 Nov 2;73(19):6663-6677. doi: 10.1093/jxb/erac328.
10
Tight regulation of the interaction between Brassica napus and Sclerotinia sclerotiorum at the microRNA level.油菜与核盘菌互作的 microRNA 水平的精细调控。
Plant Mol Biol. 2016 Sep;92(1-2):39-55. doi: 10.1007/s11103-016-0494-3. Epub 2016 Jun 20.

引用本文的文献

1
Exploring the multifaceted dynamics of flowering time regulation in field crops: Insight and intervention approaches.探索大田作物开花时间调控的多方面动态：见解与干预方法。
Plant Genome. 2025 Jun;18(2):e70017. doi: 10.1002/tpg2.70017.
2
Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.利用启动子元件增强植物基因编辑：观点与进展
Plant Biotechnol J. 2025 May;23(5):1375-1395. doi: 10.1111/pbi.14533. Epub 2025 Feb 27.
3
Antimicrobial Peptides: Classification, Mechanism, and Application in Plant Disease Resistance.

本文引用的文献

1
Cloning and Expression of a Nonribosomal Peptide Synthetase to Generate Blue Rose.用于培育蓝色玫瑰的非核糖体肽合成酶的克隆与表达
ACS Synth Biol. 2019 Aug 16;8(8):1698-1704. doi: 10.1021/acssynbio.8b00187. Epub 2018 Sep 18.
2
Mechanisms of Broad Host Range Necrotrophic Pathogenesis in Sclerotinia sclerotiorum.核盘菌广谱性坏死性致病机制研究。
Phytopathology. 2018 Oct;108(10):1128-1140. doi: 10.1094/PHYTO-06-18-0197-RVW. Epub 2018 Aug 30.
3
Plants send small RNAs in extracellular vesicles to fungal pathogen to silence virulence genes.
抗菌肽：分类、作用机制及其在植物抗病中的应用
Probiotics Antimicrob Proteins. 2025 Jun;17(3):1432-1446. doi: 10.1007/s12602-025-10478-6. Epub 2025 Feb 19.
4
Identification and expression analysis of TALE superfamily genes explore their key roles in response to abiotic stress in Brassica napus.甘蓝型油菜TALE超家族基因的鉴定与表达分析，探究其在响应非生物胁迫中的关键作用。
BMC Plant Biol. 2024 Dec 23;24(1):1238. doi: 10.1186/s12870-024-05953-1.
5
Plant Engineering to Enable Platforms for Sustainable Bioproduction of Terpenoids.植物工程助力萜类化合物可持续生物生产平台的建立。
Methods Mol Biol. 2024;2760:3-20. doi: 10.1007/978-1-0716-3658-9_1.
6
Functional and evolutionary study of MLO gene family in the regulation of Sclerotinia stem rot resistance in Brassica napus L.甘蓝型油菜中MLO基因家族在调控菌核病抗性中的功能及进化研究
Biotechnol Biofuels Bioprod. 2023 May 23;16(1):86. doi: 10.1186/s13068-023-02325-z.
7
Genome-Wide Identification and Characterization of Ammonium Transporter (AMT) Genes in Rapeseed ( L.).油菜（ L.）中铵转运蛋白（AMT）基因的全基因组鉴定和特征分析。
Genes (Basel). 2023 Mar 6;14(3):658. doi: 10.3390/genes14030658.
8
Genome-wide association study reveals a gene regulating plant height in .全基因组关联研究揭示了一个调控[植物名称]株高的基因。 （原文中未明确植物名称，这里补充了“[植物名称]”使译文更完整）
Front Plant Sci. 2022 Nov 2;13:1061196. doi: 10.3389/fpls.2022.1061196. eCollection 2022.
9
Genome-wide characterization of ovate family protein gene family associated with number of seeds per silique in .与每角果种子数相关的卵形家族蛋白基因家族的全基因组特征分析 。（注：原文中“in.”后面内容缺失）
Front Plant Sci. 2022 Sep 14;13:962592. doi: 10.3389/fpls.2022.962592. eCollection 2022.
10
The Sclerotinia sclerotiorum-inducible promoter pBnGH17D7 in Brassica napus: isolation, characterization, and application in host-induced gene silencing.甘蓝型油菜中受核盘菌诱导的启动子 pBnGH17D7 的分离、鉴定及在寄主诱导基因沉默中的应用。
J Exp Bot. 2022 Nov 2;73(19):6663-6677. doi: 10.1093/jxb/erac328.
植物通过细胞外囊泡向真菌病原体发送小 RNA，从而沉默致病基因。
Science. 2018 Jun 8;360(6393):1126-1129. doi: 10.1126/science.aar4142. Epub 2018 May 17.
4
Cross-kingdom RNA trafficking and environmental RNAi-nature's blueprint for modern crop protection strategies.跨界 RNA 转移与环境 RNAi——现代作物保护策略的自然蓝图。
Curr Opin Microbiol. 2018 Dec;46:58-64. doi: 10.1016/j.mib.2018.02.003. Epub 2018 Mar 14.
5
The high-quality genome of Brassica napus cultivar 'ZS11' reveals the introgression history in semi-winter morphotype.甘蓝型油菜品种‘ZS11’的高质量基因组揭示了半冬型形态的渗入历史。
Plant J. 2017 Nov;92(3):452-468. doi: 10.1111/tpj.13669. Epub 2017 Oct 9.
6
Functional Characterization of Promoter in Response to Abiotic Stresses by Deletion Analysis in Transgenic .通过转基因缺失分析对启动子响应非生物胁迫的功能表征
Front Plant Sci. 2017 Jul 13;8:1198. doi: 10.3389/fpls.2017.01198. eCollection 2017.
7
Production of red-flowered oilseed rape via the ectopic expression of Orychophragmus violaceus OvPAP2.通过异位表达 Orychophragmus violaceus OvPAP2 生产红花油菜。
Plant Biotechnol J. 2018 Feb;16(2):367-380. doi: 10.1111/pbi.12777. Epub 2017 Jul 26.
8
PlantTFDB 4.0: toward a central hub for transcription factors and regulatory interactions in plants.植物转录因子数据库4.0：迈向植物转录因子与调控互作的核心枢纽
Nucleic Acids Res. 2017 Jan 4;45(D1):D1040-D1045. doi: 10.1093/nar/gkw982. Epub 2016 Oct 24.
9
Isolation and Functional Validation of Salinity and Osmotic Stress Inducible Promoter from the Maize Type-II H+-Pyrophosphatase Gene by Deletion Analysis in Transgenic Tobacco Plants.通过转基因烟草植株中的缺失分析对玉米II型H⁺ - 焦磷酸酶基因盐度和渗透胁迫诱导型启动子进行分离与功能验证
PLoS One. 2016 Apr 21;11(4):e0154041. doi: 10.1371/journal.pone.0154041. eCollection 2016.
10
Low temperature-induced DNA hypermethylation attenuates expression of RhAG, an AGAMOUS homolog, and increases petal number in rose (Rosa hybrida).低温诱导的DNA高甲基化减弱了AGAMOUS同源物RhAG的表达，并增加了玫瑰（Rosa hybrida）的花瓣数量。
BMC Plant Biol. 2015 Oct 5;15:237. doi: 10.1186/s12870-015-0623-1.