Altaf Noor Ul Huda, Naz Muhammad Yasin, Shukrullah Shazia, Ghamkhar Madiha, Irfan Muhammad, Rahman Saifur, Jakubowski Tomasz, Alqurashi Esam A, Glowacz Adam, Mahnashi Mater H
Department of Physics, University of Agriculture Faisalabad, Faisalabad 38040, Pakistan.
Department of Mathematics and Statistics, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
Materials (Basel). 2022 May 27;15(11):3826. doi: 10.3390/ma15113826.
An environmentally friendly non-thermal DC plasma reduction route was adopted to reduce Ag+ ions at the plasma−liquid interface into silver nanoparticles (AgNPs) under statistically optimized conditions for biological and photocatalytic applications. The efficiency and reactivity of AgNPs were improved by statistically optimizing the reaction parameters with a Box−Behnken Design (BBD). The size of the AgNPs was chosen as a statistical response parameter, while the concentration of the stabilizer, the concentration of the silver salt, and the plasma reaction time were chosen as independent factors. The optimized parameters for the plasma production of AgNPs were estimated using a response surface methodology and a significant model p < 0.05. The AgNPs, prepared under optimized conditions, were characterized and then tested for their antibacterial, antioxidant, and photocatalytic potentials. The optimal conditions for these three activities were 3 mM of stabilizing agent, 5 mM of AgNO3, and 30 min of reaction time. Having particles size of 19 to 37 nm under optimized conditions, the AgNPs revealed a 82.3% degradation of methyl orange dye under UV light irradiation. The antibacterial response of the optimized AgNPs against S. aureus and E. coli strains revealed inhabitation zones of 15 mm and 12 mm, respectively, which demonstrate an antioxidant activity of 81.2%.
采用一种环境友好的非热直流等离子体还原途径，在生物和光催化应用的统计优化条件下，将等离子体-液体界面处的Ag⁺离子还原为银纳米颗粒（AgNPs）。通过Box-Behnken设计（BBD）对反应参数进行统计优化，提高了AgNPs的效率和反应活性。选择AgNPs的尺寸作为统计响应参数，而选择稳定剂浓度、银盐浓度和等离子体反应时间作为独立因素。使用响应面方法估计了AgNPs等离子体制备的优化参数，显著模型p < 0.05。对在优化条件下制备的AgNPs进行表征，然后测试其抗菌、抗氧化和光催化潜力。这三种活性的最佳条件是3 mM稳定剂、5 mM AgNO₃和30分钟反应时间。在优化条件下，AgNPs的粒径为19至37 nm，在紫外光照射下对甲基橙染料的降解率为82.3%。优化后的AgNPs对金黄色葡萄球菌和大肠杆菌菌株的抗菌反应分别显示出15 mm和12 mm的抑菌圈，其抗氧化活性为81.2%。
