Biomaterials Laboratory (BIOMAT), Department of Metallurgical Engineering and Materials (DEMM) and Analytical Center, Federal University of Ceará (UFC), Campus do Pici Fortaleza, CEP 60455-900, Fortaleza, CE, Brazil.
Biotechnology Laboratory, Food Engineering Department, Federal University of Ceará (UFC), Campus do Pici Fortaleza, Fortaleza, CE, Brazil.
Funct Plant Biol. 2021 Oct;48(11):1113-1123. doi: 10.1071/FP21161.
Silver nanoparticle (AgNPs) toxicity is related to nanoparticle interaction with the cell wall of microorganisms and plants. This interaction alters cell wall conformation with increased reactive oxygen species (ROS) in the cell. With the increase of ROS in the cell, the dissolution of zero silver (Ag0) to ionic silver (Ag+) occurs, which is a strong oxidant agent to the cellular wall. AgNP interaction was evaluated by transmission electron microscopy (TEM) on Lactuca sativa roots, and the mechanism of passage through the outer cell wall (OCW) was also proposed. The results suggest that Ag+ binds to the hydroxyls (OH) present in the cellulose structure, thus causing the breakdown of the hydrogen bonds. Changes in cell wall structure facilitate the passage of AgNPs, reaching the plasma membrane. According to the literature, silver nanoparticles with an average diameter of 15nm are transported across the membrane into the cells by caveolines. This work describes the interaction between AgNPs and the cell wall and proposes a transport model through the outer cell wall.
银纳米颗粒（AgNPs）的毒性与纳米颗粒与微生物和植物细胞壁的相互作用有关。这种相互作用改变了细胞壁的构象，导致细胞内活性氧（ROS）增加。随着细胞内 ROS 的增加，零价银（Ag0）溶解为离子银（Ag+），这对细胞壁是一种很强的氧化剂。通过对莴苣根进行透射电子显微镜（TEM）评估 AgNP 的相互作用，并提出了穿过细胞壁（OCW）的机制。结果表明，Ag+与纤维素结构中存在的羟基（OH）结合，从而导致氢键的破坏。细胞壁结构的变化有助于 AgNPs 通过，到达质膜。根据文献，平均直径为 15nm 的银纳米颗粒通过 caveolines 穿过膜进入细胞。这项工作描述了 AgNPs 与细胞壁的相互作用，并提出了通过细胞壁的运输模型。
