Mishra Rahul Chandra, Kalra Rishu, Dilawari Rahul, Goel Mayurika, Barrow Colin J
TERI-Deakin Nano Biotechnology Centre, The Energy and Resources Institute (TERI), TERI GRAM, Gurgaon 122001, India.
Centre for Bioprocessing, School of Life and Environmental Sciences, Deakin University, Waurn Ponds, VIC 3220, Australia.
Materials (Basel). 2022 May 29;15(11):3877. doi: 10.3390/ma15113877.
Gold nanoparticles (GNP) were bio-fabricated utilizing the methanolic extract of the endophytic isolate . The biosynthesised gold nanoparticles (GNP023) were characterised using UV-visible spectroscopy (UV-Vis); transmission electron microscopy (TEM), Fourier-transform nfrared spectroscopy (FTIR) and X-ray diffraction (XRD) studies. The bio-fabricated GNP023 displayed a sharp SPR peak at 536 nm, were spherically shaped, and had an average size between 10-16 nm. The EDX profile confirmed the presence of gold (Au), and XRD analysis confirmed the crystalline nature of GNP023. The antimicrobial activity of GNP023 was investigated against several food-borne and phytopathogens, using in vitro antibacterial and antifungal assays. The maximum zone of inhibition was observed for and at 400 μg /mL, whereas inhibition in radial mycelial growth was observed against and at 52.5% and 65.46%, respectively, when challenged with GNP023 (200 μg/mL). Moreover, the gold nanoparticles displayed significant antioxidant activity against the ABTS radical, with an IC of 38.61 µg/mL, and were non-toxic when tested against human kidney embryonic 293 (HEK293) cells. Thus, the current work supports the application of myco-synthesised gold nanoparticles as a versatile antimicrobial candidate against food-borne pathogens.
利用内生分离物的甲醇提取物生物合成了金纳米颗粒（GNP）。使用紫外可见光谱（UV-Vis）、透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）研究对生物合成的金纳米颗粒（GNP023）进行了表征。生物合成的GNP023在536nm处显示出尖锐的表面等离子体共振（SPR）峰，呈球形，平均尺寸在10-16nm之间。能谱分析（EDX）证实了金（Au）的存在，XRD分析证实了GNP023的晶体性质。使用体外抗菌和抗真菌试验研究了GNP023对几种食源性病原体和植物病原体的抗菌活性。在400μg/mL时，观察到对[具体菌种1]和[具体菌种2]的最大抑菌圈，而当用GNP023（200μg/mL）攻击时，观察到对[具体菌种3]和[具体菌种4]的径向菌丝生长抑制率分别为52.5%和65.46%。此外，金纳米颗粒对ABTS自由基显示出显著的抗氧化活性，IC50为38.61μg/mL，并且在针对人肾胚胎293（HEK293）细胞进行测试时无毒。因此，目前的工作支持将真菌合成的金纳米颗粒作为一种通用的抗食源性病原体抗菌候选物的应用。
