Department of Plant Protection, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman 7616914111, Iran.
Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad 9177948978, Iran.
Molecules. 2022 Feb 10;27(4):1181. doi: 10.3390/molecules27041181.
The aim of our study was to examine the different concentrations of AuNPs as a new antimicrobial substance to control the pathogenic activity. The extracellular synthesis of AuNPs performed by using sp. as an endophytic fungus. Endophytic fungus was isolated from vascular tissue of peach trees () from Baft, located in Kerman province, Iran. The UltraViolet-Visible Spectroscopy (UV-Vis spectroscopy) and Fourier transform infrared spectroscopy provided the absorbance peak at 526 nm, while the X-ray diffraction and transmission electron microscopy images released the formation of spherical AuNPs with sizes in the range of 10-100 nm. The findings of inhibition zone test of Au nanoparticles (AuNPs) showed a desirable antifungal and antibacterial activity against phytopathogens including AG1-IA (AG1-IA has been identified as the dominant anastomosis group) and pv. . The highest inhibition level against sclerotia formation was 93% for AuNPs at a concentration of 80 μg/mL. Application of endophytic fungus biomass for synthesis of AuNPs is relatively inexpensive, single step and environmentally friendly. In vitro study of the antifungal activity of AuNPs at concentrations of 10, 20, 40 and 80 μg/mL was conducted against rice fungal pathogen to reduce sclerotia formation. The experimental data revealed that the Inhibition rate (RH) for sclerotia formation was (15, 33, 74 and 93%), respectively, for their corresponding AuNPs concentrations (10, 20, 40 and 80 μg/mL). Our findings obviously indicated that the RH strongly depend on AuNPs rates, and enhance upon an increase in AuNPs rates. The application of endophytic fungi biomass for green synthesis is our future goal.
我们的研究目的是检验不同浓度的 AuNPs 作为一种新的抗菌物质来控制致病活性。通过 sp. 作为内生真菌来进行 AuNPs 的细胞外合成。内生真菌是从伊朗克尔曼省巴夫特的桃树维管束组织中分离出来的。紫外可见光谱（UV-Vis 光谱）和傅里叶变换红外光谱在 526nm 处提供了吸收峰，而 X 射线衍射和透射电子显微镜图像则显示出形成了直径在 10-100nm 范围内的球形 AuNPs。Au 纳米粒子（AuNPs）抑菌圈试验的结果表明，对包括 AG1-IA（AG1-IA 已被确定为主要吻合组）和 pv. 在内的植物病原菌具有良好的抗真菌和抗菌活性。AuNPs 在 80μg/mL 浓度下对菌核形成的最高抑制水平为 93%。内生真菌生物量用于合成 AuNPs 的方法相对便宜、单步和环保。在体外研究了 10、20、40 和 80μg/mL 浓度的 AuNPs 对水稻真菌病原体 的抗真菌活性，以减少菌核形成。实验数据表明，菌核形成的抑制率（RH）分别为 15%、33%、74%和 93%，对应于它们各自的 AuNPs 浓度（10、20、40 和 80μg/mL）。我们的研究结果明显表明，RH 强烈依赖于 AuNPs 的浓度，并随着 AuNPs 浓度的增加而增强。内生真菌生物量的绿色合成是我们未来的目标。
