Takeda N, Niwa T, Tatematsu A, Suzuki M
Clin Chem. 1987 May;33(5):682-5.
A major protein-bound ligand in the serum of chronic hemodialysis patients was isolated from heat-deproteinized uremic serum by "high-performance" liquid chromatography (HPLC). The isolated compound was identified as 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid by use of liquid secondary-ion mass spectrometry and gas chromatography-mass spectrometry. On HPLC, an authentic sample of this compound showed a retention time identical to that of the protein-bound ligand peak. The concentration of the furancarboxylic acid in serum, as estimated by HPLC, is markedly greater in chronic hemodialysis patients than in normal subjects.
通过“高效”液相色谱法（HPLC）从热脱蛋白的尿毒症血清中分离出慢性血液透析患者血清中的一种主要蛋白质结合配体。利用液相二次离子质谱法和气相色谱 - 质谱法将分离出的化合物鉴定为3 - 羧基 - 4 - 甲基 - 5 - 丙基 - 2 - 呋喃丙酸。在HPLC上，该化合物的纯品样品显示出与蛋白质结合配体峰相同的保留时间。通过HPLC估计，慢性血液透析患者血清中呋喃羧酸的浓度明显高于正常受试者。
