Ding Zhou, He Yue, Rao Hongtao, Zhang Le, Nguyen William, Wang Jingjing, Wu Ying, Han Caiqin, Xing Christina, Yan Changchun, Chen Wei, Liu Ying
Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, China.
Department of Physics, The University of Texas at Arlington, Arlington, TX 76019-0059, USA.
Nanomaterials (Basel). 2022 May 24;12(11):1787. doi: 10.3390/nano12111787.
In this paper, a novel rare-earth-doped upconverted nanomaterial NaYF:Yb,Tm fluorescent probe is reported, which can detect cancer-related specific miRNAs in low abundance. The detection is based on an upconversion of nanomaterials NaYF:Yb,Tm, with emissions at 345, 362, 450, 477, 646, and 802 nm, upon excitation at 980 nm. The optimal Yb:Tm doping ratio is 40:1, in which the NaYF:Yb,Tm nanomaterials have the strongest fluorescence. The NaYF:Yb, Tm nanoparticles were coated with carboxylation or carboxylated protein, in order to improve their water solubility and biocompatibility. The two commonly expressed proteins, miRNA-155 and miRNA-150, were detected by the designed fluorescent probe. The results showed that the probes can distinguish miRNA-155 well from partial and complete base mismatch miRNA-155, and can effectively distinguish miRNA-155 and miRNA-150. The preliminary results indicate that these upconverted nanomaterials have good potential for protein detection in disease diagnosis, including early cancer detection.
本文报道了一种新型的稀土掺杂上转换纳米材料NaYF:Yb,Tm荧光探针，它能够检测低丰度的癌症相关特异性微小RNA。该检测基于纳米材料NaYF:Yb,Tm的上转换，在980nm激发下，其发射波长为345、362、450、477、646和802nm。最佳的Yb:Tm掺杂比例为40:1，此时NaYF:Yb,Tm纳米材料具有最强的荧光。NaYF:Yb,Tm纳米颗粒用羧基化或羧基化蛋白进行包覆，以提高其水溶性和生物相容性。通过设计的荧光探针检测了两种常见表达的蛋白质，即微小RNA-155和微小RNA-150。结果表明，该探针能够很好地区分微小RNA-155与部分和完全碱基错配的微小RNA-155，并且能够有效地区分微小RNA-155和微小RNA-150。初步结果表明，这些上转换纳米材料在包括早期癌症检测在内的疾病诊断中的蛋白质检测方面具有良好的潜力。
