Hassanin Hanaa A, Taha Amel
Department of Chemistry, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi Arabia.
Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo 11566, Egypt.
Nanomaterials (Basel). 2022 Jun 3;12(11):1919. doi: 10.3390/nano12111919.
Theophrasite β-Ni(OH) nanocluster were fabricated via the sonochemical-assisted biogenic method using chia seeds extract as a reducing and stabilizing agent. The optical and morphological feature of the synthesized nanocluster was characterized using UV-Vis, FTIR, FE-SEM-EDS, HR-TEM, DLS, XPS, and XRD analysis. According to FE-SEM and HR-TEM images of the synthesized materials, β-Ni(OH) nanocluster illustrates the hexagonal particle shape with an average size of 5.8 nm, while the EDS results confirm the high purity of the synthesized nanocluster. Moreover, the XRD pattern of the synthesized materials shows typical peaks that match the reference pattern of the Theophrasite form of β-Ni(OH) with a hexagonal crystal system. The XPS analysis illustrates that the prepared samples exhibit both Ni and Ni with the predominance of Ni species. Additionally the in-vitro cytotoxic activity of β-Ni(OH) nanocluster is tested against the MCF7 cell lines (breast cancer cells). The MTT assay results proved that the synthesized β-Ni(OH) nanocluster has potent cytotoxic activity against breast cancer cell lines (IC: 62.7 μg/mL).
利用奇亚籽提取物作为还原剂和稳定剂，通过声化学辅助生物方法制备了硫砷镍矿型β-Ni(OH)纳米团簇。使用紫外可见光谱、傅里叶变换红外光谱、场发射扫描电子显微镜-能谱仪、高分辨率透射电子显微镜、动态光散射、X射线光电子能谱和X射线衍射分析对合成的纳米团簇的光学和形态特征进行了表征。根据合成材料的场发射扫描电子显微镜和高分辨率透射电子显微镜图像，β-Ni(OH)纳米团簇呈现六边形颗粒形状，平均尺寸为5.8纳米，而能谱仪结果证实了合成的纳米团簇的高纯度。此外，合成材料的X射线衍射图谱显示出与六方晶系硫砷镍矿型β-Ni(OH)的参考图谱相匹配的典型峰。X射线光电子能谱分析表明，制备的样品同时呈现Ni和Ni，其中Ni物种占主导。此外，还测试了β-Ni(OH)纳米团簇对MCF7细胞系（乳腺癌细胞）的体外细胞毒性活性。MTT法测定结果证明，合成的β-Ni(OH)纳米团簇对乳腺癌细胞系具有强大的细胞毒性活性（IC：62.7μg/mL）。
