Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute (CNCI), 37, S.P. Mukherjee Road, Kolkata 700026, West Bengal, India.
Colloids Surf B Biointerfaces. 2018 Jan 1;161:111-120. doi: 10.1016/j.colsurfb.2017.10.040. Epub 2017 Oct 13.
In the present study, we demonstrate a simple, cost-effective and eco-friendly method for biogenic synthesis of silver nanoparticles (AgNPCGs) using ethanolic extract of Calotropis gigantea latex. Attempts were made to characterize these biogenic silver nanoparticles AgNPCGs and also to test its cytotoxic, anti-neoplastic and apoptotic potential through the induction of oxidative stress, mitochondrial dysfunction. AgNPCGs were characterized by UV-vis spectroscopy, dynamic light scattering (DLS) and surface zeta potential measurement, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and selected area electron diffraction, scanning electron microscopy (SEM), energy-dispersive X-ray fluorescence spectrometry (EDX). UV visible spectroscopy showed an intense surface plasmon resonance band at 431nm which clearly reflected the formation of silver nanoparticles. FTIR study revealed that latex extract acted as reducing and stabilizing agent for the synthesis of AgNPCGs. Energy dispersive X-ray spectroscopy confirmed the presence of silver as a major component of synthesized AgNPCGs. SEM and TEM studies showed that the synthesized AgNPCGs were nearly spherical in shape with an average size of 2.338nm. The selected area electron diffraction pattern and XRD studies confirmed the crystalline nature of AgNPCGs. AgNPCGs exhibited in-vitro cytotoxic activity against Ehrlich's ascites carcinoma (EAC), Jurkat and MCF-7 cells at respective IC doses without producing cytotoxicity to mice and human lymphocytes. Significant chromatin condensation, DNA fragmentation, cell cycle arrest at G/M phase, up-regulation of Bax and caspase-3 and down-regulation of Bcl-2 were observed in AgNPCGs treated EAC cells. The results suggest that biogenic silver nanoparticles AgNPCGs could be a potential chemotherapeutic formulation for cancer therapy.
在本研究中，我们展示了一种使用大猪草乳胶乙醇提取物生物合成银纳米颗粒（AgNPCGs）的简单、经济高效且环保的方法。我们试图对这些生物合成的银纳米颗粒 AgNPCGs 进行表征，并通过诱导氧化应激、线粒体功能障碍来测试其细胞毒性、抗肿瘤和凋亡潜能。AgNPCGs 通过紫外-可见光谱、动态光散射（DLS）和表面zeta 电位测量、傅里叶变换红外（FTIR）光谱、X 射线衍射（XRD）、透射电子显微镜（TEM）和选区电子衍射、扫描电子显微镜（SEM）、能量色散 X 射线荧光光谱（EDX）进行了表征。紫外可见光谱在 431nm 处显示出强烈的表面等离子体共振带，清楚地反映了银纳米颗粒的形成。FTIR 研究表明，乳胶提取物在 AgNPCGs 的合成中既起到了还原作用，也起到了稳定作用。能谱分析证实了银是合成的 AgNPCGs 的主要成分。SEM 和 TEM 研究表明，合成的 AgNPCGs 呈近球形，平均粒径为 2.338nm。选区电子衍射图案和 XRD 研究证实了 AgNPCGs 的结晶性质。AgNPCGs 在体外对艾氏腹水癌（EAC）、Jurkat 和 MCF-7 细胞表现出细胞毒性，在相应的 IC 剂量下没有对小鼠和人淋巴细胞产生细胞毒性。在 AgNPCGs 处理的 EAC 细胞中观察到明显的染色质浓缩、DNA 片段化、细胞周期停滞在 G/M 期、Bax 和 caspase-3 上调以及 Bcl-2 下调。结果表明，生物合成的银纳米颗粒 AgNPCGs 可能是一种有前途的癌症治疗化疗制剂。
