Sukkho Treethip, Khanongnuch Chartchai, Lumyong Saisamorn, Ruangsuriya Jetsada, Pattananandecha Thanawat, Apichai Sutasinee, Ogata Fumihiko, Kawasaki Naohito, Saenjum Chalermpong
Department of Biotechnology, Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand.
Center of Excellence for Innovation in Analytical Science and Technology for Biodiversity-Based Economic and Society (I-ANALY-S-T_B.BES-CMU), Chiang Mai University, Chiang Mai 50200, Thailand.
Plants (Basel). 2022 Jun 1;11(11):1492. doi: 10.3390/plants11111492.
"People-Forest-Miang" communities are villages located in the cultivated area of var. , or Cha Miang, in northern Thailand. Cha Miang forests are a form of agriculture relying on forest-rich bioresources. This study focuses on a survey of the diversity of medicinal plants used by "People-Forest-Miang" communities in Mae Kampong Village, Chiang Mai, Thailand. The results demonstrated that 73 species of medicinal plants were used to prevent and treat various ailments. The highest number of species (30.14%) was used for musculoskeletal system disorders, followed by digestive system disorders (21.92%) and unspecified medicinal disorders (15.07%). The alkaline phosphatase (ALP) is the most widely recognized biochemical marker for osteoblast activity. The ALP activity of ethanol and deionized water extracts of the nine selected medicinal plants used for musculoskeletal system disorders were examined in the MG63 cell line. The results showed that the numerous water extracts, including MKP1, MKP2, MKP5, MKP6, MKP7, MKP8, and MKP9, and the ethanolic extracts-namely, MKP2, MKP3, MKP7, and MKP9-significantly increased ALP activity in the MG-63 cell line. The findings indicate that some medicinal plants may be further studied for active chemicals and developed as natural active pharmaceutical ingredients for osteoprotective products.
“人-森林-绵”社区是位于泰国北部绵变种（即差绵）种植区的村庄。差绵森林是一种依赖丰富森林生物资源的农业形式。本研究重点调查了泰国清迈湄康蓬村“人-森林-绵”社区使用的药用植物多样性。结果表明，73种药用植物被用于预防和治疗各种疾病。使用种类最多的是用于肌肉骨骼系统疾病的植物（占30.14%），其次是消化系统疾病（占21.92%）和未明确的药用疾病（占15.07%）。碱性磷酸酶（ALP）是成骨细胞活性最广为人知的生化标志物。在MG63细胞系中检测了用于肌肉骨骼系统疾病的9种选定药用植物的乙醇提取物和去离子水提取物的ALP活性。结果表明，许多水提取物，包括MKP1、MKP2、MKP5、MKP6、MKP7、MKP8和MKP9以及乙醇提取物，即MKP2、MKP3、MKP7和MKP9，均显著提高了MG-63细胞系中的ALP活性。研究结果表明，一些药用植物可能需要进一步研究其活性化学成分，并开发成为用于骨保护产品的天然活性药物成分。
