Yang Haiyan, Sun Ming, Lin Shuangji, Guo Yanhong, Yang Yongjuan, Zhang Tengxun, Zhang Jingxing
College of Landscape Architecture, Beijing Forestry University, Beijing, China.
Beijing Key Laboratory of Ornamental Plant Germplasm Innovation & Molecular Breeding, Beijing, China.
PLoS One. 2017 Nov 13;12(11):e0187124. doi: 10.1371/journal.pone.0187124. eCollection 2017.
Soil salinization is becoming a limitation to the utilization of ornamental plants worldwide. Crossostephium chinensis (Linnaeus) Makino is often cultivated along the southeast coast of China for its desirable ornamental qualities and high salt tolerance. However, little is known about the genomic background of the salt tolerance mechanism in C. chinensis. In the present study, we used Illumina paired-end sequencing to systematically investigate leaf transcriptomes derived from C. chinensis seedlings grown under normal conditions and under salt stress. A total of 105,473,004 bp of reads were assembled into 163,046 unigenes, of which 65,839 (40.38% of the total) and 54,342 (33.32% of the total) were aligned in Swiss-Prot and Nr protein, respectively. A total of 11,331 (6.95%) differentially expressed genes (DEGs) were identified among three comparisons, including 2,239 in 'ST3 vs ST0', 5,880 in 'ST9 vs ST3' and 9,718 in 'ST9 vs ST0', and they were generally classified into 26 Gene Ontology terms and 58 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway terms. Many genes encoding important transcription factors (e.g., WRKY, MYB, and AP2/EREBP) and proteins involved in starch and sucrose metabolism, arginine and proline metabolism, plant hormone signal transduction, amino acid biosynthesis, plant-pathogen interactions and carbohydrate metabolism, among others, were substantially up-regulated under salt stress. These genes represent important candidates for studying the salt-response mechanism and molecular biology of C. chinensis and its relatives. Our findings provide a genomic sequence resource for functional genetic assignments in C. chinensis. These transcriptome datasets will help elucidate the molecular mechanisms responsible for salt-stress tolerance in C. chinensis and facilitate the breeding of new stress-tolerant cultivars for high-saline areas using this valuable genetic resource.
土壤盐渍化正成为全球观赏植物利用的一个限制因素。芙蓉菊（Crossostephium chinensis (Linnaeus) Makino）因其优良的观赏品质和较高的耐盐性，常被种植于中国东南沿海地区。然而，关于芙蓉菊耐盐机制的基因组背景却知之甚少。在本研究中，我们利用Illumina双末端测序技术，系统地研究了芙蓉菊幼苗在正常条件和盐胁迫下的叶片转录组。总共105,473,004 bp的reads被组装成163,046个单基因，其中分别有65,839个（占总数的40.38%）和54,342个（占总数的33.32%）与Swiss-Prot和Nr蛋白比对。在三个比较组中总共鉴定出11,331个（6.95%）差异表达基因（DEGs），包括“ST3 vs ST0”中的2,239个、“ST9 vs ST3”中的5,880个和“ST9 vs ST0”中的9,718个，它们通常被归类为26个基因本体论术语和58个京都基因与基因组百科全书（KEGG）通路术语。许多编码重要转录因子（如WRKY、MYB和AP2/EREBP）的基因以及参与淀粉和蔗糖代谢、精氨酸和脯氨酸代谢、植物激素信号转导、氨基酸生物合成、植物-病原体相互作用和碳水化合物代谢等的蛋白质，在盐胁迫下显著上调。这些基因是研究芙蓉菊及其近缘种盐响应机制和分子生物学的重要候选基因。我们的研究结果为芙蓉菊功能基因定位提供了基因组序列资源。这些转录组数据集将有助于阐明芙蓉菊耐盐胁迫的分子机制，并利用这一宝贵的遗传资源促进高盐地区新的耐胁迫品种的培育。
