School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
Guangzhou Jinan Biomedicine Research and Development Center, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Molecules. 2022 May 24;27(11):3365. doi: 10.3390/molecules27113365.
The iridoid compounds in traditional Chinese medicine play a prominent role in their antiviral effects. We previously reported the anti-inflammatory effect of new iridoids from the aerial parts of . Nevertheless, several open questions remain to explore the other biological functions of these new iridoid compounds. Herpes simplex virus-1 (HSV-1) is one of the most prevalent pathogens in human beings worldwide and due to limited therapies, mainly with the guanosine analog aciclovir (ACV) and other analogs, the search for new drugs with different modes of action and low toxicity becomes particularly urgent for public health. This study aimed to explore the anti-HSV-1 effects of iridoids from the aerial parts of . The dried aerial parts of were extracted with 95% ethanol and systematic separation and purification were then carried out by modern column chromatography methods such as silica gel column, RP-ODS column, Sephadex LH-20 gel column, and semi-preparative liquid phase, and the structure of these compounds were identified through the physical and chemical properties and a variety of spectral techniques. The obtained seven new iridoid compounds were screened for antiviral activity on HSV-1 through CCK8 and the cytopathic effect, and then the plaque reduction assay, the anti-fluorescence reporter virus strain replication, and RT-qPCR experiments were carried out to further evaluate the antiviral effect. Seven new iridoid compounds (officinaloside A-G) were identified from the aerial parts of , and officinaloside C showed anti-HSV-1 activity. Further functional experiments confirmed that officinaloside C has a significant inhibiting effect on HSV-1 virus plaque formation, viral gene, and protein expression, and fluorescent virus replication. Our findings suggest that officinaloside C has significant inhibitory effects on viral plaque formation, genome replication, and viral protein expression of HSV-1 which implies that officinaloside C exhibits viral activity and may be a promising treatment for HSV-1 infection.
中药中的环烯醚萜类化合物在其抗病毒作用中起着突出的作用。我们之前报道了从地上部分中新的环烯醚萜类化合物的抗炎作用。然而，仍有一些悬而未决的问题需要探索这些新的环烯醚萜化合物的其他生物学功能。单纯疱疹病毒-1 (HSV-1) 是全球人类最常见的病原体之一，由于治疗方法有限，主要是使用鸟嘌呤类似物阿昔洛韦 (ACV) 和其他类似物，因此迫切需要寻找具有不同作用模式和低毒性的新药，这对公共卫生尤为重要。本研究旨在探索地上部分中环烯醚萜类化合物对 HSV-1 的抗作用。采用 95%乙醇对 的干燥地上部分进行提取，然后通过现代柱层析方法，如硅胶柱、反相-ODS 柱、Sephadex LH-20 凝胶柱和半制备液相进行系统分离和纯化，并通过理化性质和多种光谱技术鉴定这些化合物的结构。通过 CCK8 和细胞病变效应筛选出这七种新的环烯醚萜化合物对 HSV-1 的抗病毒活性，然后进行噬斑减少试验、抗荧光报告病毒株复制和 RT-qPCR 实验，进一步评价抗病毒作用。从地上部分中鉴定出 7 种新的环烯醚萜化合物（栀子苷 A-G），栀子苷 C 显示出抗 HSV-1 的活性。进一步的功能实验证实，栀子苷 C 对 HSV-1 病毒斑形成、病毒基因和蛋白表达以及荧光病毒复制有显著的抑制作用。我们的研究结果表明，栀子苷 C 对 HSV-1 病毒斑形成、基因组复制和病毒蛋白表达有显著的抑制作用，这表明栀子苷 C 具有抗病毒活性，可能是治疗 HSV-1 感染的一种有前途的方法。
