Rouveix B, Groult F, Pocidalo J J
Clin Exp Immunol. 1986 Dec;66(3):574-81.
A study was made to evaluate the leukocyte aggregation test (LAT) in patients at risk for acquired immunodeficiency syndrome (AIDS) and with confirmed AIDS using tuberculin, streptococcus and candida as recall antigens. These patients were selected on the basis of their well known absence of delayed hypersensitivity, a fact which should avoid false positive tests. Antigen-induced human peripheral blood leukocyte aggregation was measured quantitatively. The results obtained have been compared with the leukocyte migration inhibition test (LMIT) in patient and control groups. Among the 46 patients, less than 7% were positive for the LAT, whereas more than 50% were LMIT positive (P less than 0.001). These results suggest that there are fewer false positive reactions with LAT which can therefore be considered as a reliable method for assessing CMI in human. Furthermore, a negative LAT correlated well with negative delayed skin tests in more than 90% of the patients for the three antigens. These patients had either an AIDS or an advanced AIDS-related complex (ARC). In only eight of the patients was there a discrepancy in the results of the two tests. A positive LAT and a negative skin test were seen in three cases lacking opportunistic infections (OI), whereas a negative LAT and a positive skin test were found in patients with or without OI. In the latter, a negative LAT could indicate a more advanced stage of disease and hence a poor prognosis.
一项研究旨在评估使用结核菌素、链球菌和念珠菌作为回忆抗原，对获得性免疫缺陷综合征（AIDS）高危患者及确诊AIDS患者进行白细胞聚集试验（LAT）的情况。这些患者是基于其众所周知的缺乏迟发型超敏反应而入选的，这一事实应可避免假阳性试验。定量测量抗原诱导的人外周血白细胞聚集情况。将所得结果与患者组和对照组的白细胞迁移抑制试验（LMIT）进行了比较。在46例患者中，LAT阳性率低于7%，而LMIT阳性率超过50%（P<0.001）。这些结果表明，LAT的假阳性反应较少，因此可被视为评估人体细胞介导免疫（CMI）的可靠方法。此外，在超过90%的患者中，对于三种抗原，LAT阴性与皮肤迟发型试验阴性密切相关。这些患者患有AIDS或晚期AIDS相关综合征（ARC）。只有8例患者两项试验结果存在差异。在3例无机会性感染（OI）的患者中出现LAT阳性而皮肤试验阴性，而在有或无OI的患者中发现LAT阴性而皮肤试验阳性。在后者中，LAT阴性可能表明疾病处于更晚期，因此预后较差。
