Genome-Wide Analysis of the Cis-Prenyltransferase (CPT) Gene Family in Provides Insights into Its Expression Patterns in Response to Hormonal Treatments.

(TKS) is a natural rubber (NR)-producing plant with great development prospects. Accurately understanding the molecular mechanism of natural rubber biosynthesis is of great significance. Cis-prenyltransferase (CPT) and cis-prenyltransferase-like (CPTL) proteins catalyze the elongation of natural rubber molecular chains and play an essential role in rubber biosynthesis. In this study, we performed a genome-wide identification of the / family, with eight and two members. We analyzed the gene structures, evolutionary relationships and expression patterns, revealing five highly conserved structural domains. Based on systematic evolutionary analysis, CPT/CPTL can be divided into six subclades, among which the family members are most closely related to the orthologous species Collinearity analyses showed that fragment duplications were the primary factor of amplification in the gene family. Induced by ethylene and methyl jasmonate hormones, the expression levels of most genes increased, with significant increases in the expression levels of and . Our results provide a theoretical basis for elucidating the role of the / gene family in the mechanism of natural rubber synthesis and lay a foundation for molecular breeding of and candidate genes for regulating natural rubber biosynthesis in the future.