College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Molecules. 2022 Jul 29;27(15):4866. doi: 10.3390/molecules27154866.
is a perennial epiphytic herb in Orchidaceae. Cultivated products are the main alternative for clinical application due to the shortage of wild resources. However, the phenotype and quality of have changed post-artificial cultivation, and environmental cues such as light, temperature, water, and nutrition supply are the major influencing factors. This study aims to unveil the mechanisms beneath the cultivation-induced variation by analyzing the changes of the metabolome and transcriptome of seedlings treated with red- blue LED light and potassium fertilizer.
After light- and K-treatment, the pseudobulbs turned purple and the anthocyanin content increased significantly. Through wide-target metabolome analysis, compared with pseudobulbs in the control group (P), the proportion of flavonoids in differentially-accumulated metabolites (DAMs) was 22.4% and 33.5% post light- and K-treatment, respectively. The gene modules coupled to flavonoids were obtained through the coexpression analysis of the light- and K-treated transcriptome by WGCNA. The KEGG enrichment results of the key modules showed that the DEGs of the pseudobulb were enriched in phenylpropane biosynthesis, flavonoid biosynthesis, and jasmonic acid (JA) synthesis post-light- and K-treatment. In addition, anthocyanin accumulation was the main contribution to the purple color of pseudobulbs, and the plant hormone JA induced the accumulation of anthocyanins in .
These results suggested that light and potassium affected the accumulation of active compounds in , and the gene-flavone network analysis emphasizes the key functional genes and regulatory factors for quality improvement in the cultivation of this medicinal plant.
是兰科的一种多年生附生草本植物。由于野生资源短缺，栽培产品是临床应用的主要替代品。然而，经过人工栽培后，的表型和品质发生了变化，光、温度、水和营养供应等环境线索是主要的影响因素。本研究旨在通过分析红蓝 LED 光和钾肥处理的幼苗代谢组和转录组的变化，揭示栽培诱导变异的机制。
光和 K 处理后，假鳞茎变紫，花色苷含量显著增加。通过广泛靶向代谢组分析，与对照组（P）相比，光和 K 处理后差异积累代谢物（DAMs）中类黄酮的比例分别为 22.4%和 33.5%。通过 WGCNA 对光和 K 处理的转录组进行共表达分析，获得了与类黄酮相关的基因模块。关键模块的 KEGG 富集结果表明，光和 K 处理后，的假鳞茎 DEGs 富集于苯丙烷生物合成、类黄酮生物合成和茉莉酸（JA）合成。此外，花色苷的积累是假鳞茎变紫的主要原因，植物激素 JA 诱导花色苷在中的积累。
这些结果表明，光照和钾影响了在中的活性化合物的积累，基因-黄酮网络分析强调了该药用植物栽培中提高品质的关键功能基因和调控因子。
