Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Horus University in Egypt, New Damietta 34517, Egypt.
Molecules. 2022 Jun 6;27(11):3639. doi: 10.3390/molecules27113639.
Unprecedented nanoemulsion formulations (NE) of and essential oils (EO) were prepared, and examined for their cytotoxic and antiviral activities. NE characterization and stability examination tests were performed to ensure formula stability. The antiviral activity was determined against hepatitis A (HAV) and herpes simplex type-1 (HSV-1) viruses using MTT assay, while the cytotoxic potential was determined against liver (HepG-2), breast (MCF-7), leukemia (THP-1) cancer cell lines and normal Vero cells. Statistical significance was determined in comparison with doxorubicin as cytotoxic and acyclovir as antiviral standard drugs. GC-MS analysis indicated twenty four compounds in the EO of and seventeen compounds in the EO of . Biological investigations of pure EOs revealed weak cytotoxic and antiviral effects. Nevertheless, their NE formulations exhibited high biological value as cytotoxic and antiviral agents. NE formulations also showed feasible selectivity index for the viral-infected and cancer cells (especially HepG-2) than normal Vero cells. Both nanoemulsions showed lower IC than standard doxorubicin against HepG-2 (26.65 and 22.58 vs. 33.96 μg/mL) and MCF-7 (36.09 and 36.19 vs. 52.73 μg/mL), respectively. The study results showed the dramatic effect of nanoemulsion preparation on the biological activity of EOs and other liposoluble phytopharmaceuticals.
制备了 和 精油（EO）的前所未有的纳米乳剂（NE）制剂，并研究了它们的细胞毒性和抗病毒活性。进行了 NE 特征和稳定性检查测试，以确保配方的稳定性。使用 MTT 测定法测定了针对甲型肝炎（HAV）和单纯疱疹病毒-1（HSV-1）病毒的抗病毒活性，而细胞毒性潜力则针对肝癌（HepG-2），乳腺癌（MCF-7），白血病（THP-1）癌细胞系和正常 Vero 细胞进行了测定。与阿霉素作为细胞毒性和阿昔洛韦作为抗病毒标准药物进行了比较，确定了统计学意义。GC-MS 分析表明， 和 的精油中分别有二十四种和十七种化合物。纯精油的生物学研究表明其具有较弱的细胞毒性和抗病毒作用。然而，它们的 NE 制剂表现出作为细胞毒性和抗病毒剂的高生物学价值。NE 制剂还显示出针对病毒感染细胞和癌细胞（尤其是 HepG-2）的可行选择性指数，而对正常 Vero 细胞则具有较低的选择性指数。两种纳米乳剂对 HepG-2（分别为 26.65 和 22.58 与 33.96 μg/mL）和 MCF-7（分别为 36.09 和 36.19 与 52.73 μg/mL）的 IC 均低于标准阿霉素。研究结果表明，纳米乳液制备对精油和其他脂溶性植物药的生物活性具有显著影响。
