Department of Pharmacology, H.K.E. Society's Matoshree Taradevi Rampure Institute of Pharmaceutical Sciences, Gulbarga, Karnataka, India.
J Cancer Res Ther. 2021 Jan-Mar;17(1):62-68. doi: 10.4103/jcrt.JCRT_915_17.
The main objective of the study was to evaluate the cytotoxicity of selected essential oils on human skin, gastric, and brain cancer cell lines using microculture tetrazolium test.
Phytochemical analysis, as well as acute oral toxicity tests, was carried out in female albino mice with cardamom oil, lemon oil, and jasmine oil according to the Organization for Economic Co-operation and Development guidelines 425. Anticancer activities of the above test drugs were performed using human cancer cell lines. The studies were carried out at Skanda Life Sciences Pvt. Ltd., Bengaluru.
Phytochemical analysis has shown the presence of carbohydrates and flavonoids in cardamom oil. While lemon oil has shown the presence of carbohydrates, flavonoids, steroids, terpenoids, and tannins, jasmine oil has shown the presence of carbohydrates, alkaloids, flavonoids, steroids, terpenoids, and glycosides. Toxicity studies showed that cardamom oil, lemon oil, and jasmine oil were all found to be safe up to 2000 mg/kg body weight. Results have shown that lemon oil exhibited the strongest cytotoxicity toward three human cancer cell lines, namely skin cancer (A431), gastric cancer (MKN-45), and brain cancer (U-87 MG) cell lines, with higher IC values of 62.82 μg/ml, 220.9 μg/ml, and 440.1 μg/ml compared to standard. Jasmine oil exhibited the strongest cytotoxicity toward skin cancer and brain cancer cell lines, whereas cardamom oil has shown stronger cytotoxicity only toward skin cancer cell line but did not show any level of inhibition of growth of brain and gastric cancer cells.
Our study reveals that lemon oil, jasmine oil, and cardamom oil possess potent antitumor activity compared to standard. At different concentrations, lemon oil has shown statistically significant (***P < 0.0001) anticancer activity toward all the three human cancer cell lines. While jasmine oil has shown statistically significant (***P < 0.0001) anticancer activity toward skin and brain cancer cell line, cardamom oil has also shown statistically significant (***P < 0.0001) anticancer activity but only toward skin cancer cell line.
本研究的主要目的是使用微量细胞培养噻唑蓝试验评估选定的精油对人皮肤、胃和脑癌细胞系的细胞毒性。
根据经济合作与发展组织 425 号指南，对豆蔻油、柠檬油和茉莉油进行植物化学分析和急性口服毒性试验，以雌性白化小鼠为实验对象。使用人癌细胞系进行上述试验药物的抗癌活性研究。该研究在班加罗尔的 Skanda Life Sciences Pvt. Ltd. 进行。
植物化学分析显示豆蔻油中含有碳水化合物和类黄酮。柠檬油含有碳水化合物、类黄酮、类固醇、萜类化合物和单宁，而茉莉油含有碳水化合物、生物碱、类黄酮、类固醇、萜类化合物和糖苷。毒性研究表明，豆蔻油、柠檬油和茉莉油的最大耐受量均在 2000mg/kg 体重以上。结果表明，柠檬油对三种人癌细胞系（皮肤癌（A431）、胃癌（MKN-45）和脑癌（U-87 MG））的细胞毒性最强，其 IC 值分别为 62.82μg/ml、220.9μg/ml 和 440.1μg/ml，均明显高于标准品。茉莉油对皮肤癌和脑癌细胞系的细胞毒性最强，而豆蔻油仅对皮肤癌细胞系表现出较强的细胞毒性，而对脑癌和胃癌细胞无抑制生长作用。
我们的研究表明，与标准品相比，柠檬油、茉莉油和豆蔻油具有更强的抗肿瘤活性。在不同浓度下，柠檬油对所有三种人癌细胞系均表现出统计学显著（***P < 0.0001）的抗癌活性。茉莉油对皮肤癌和脑癌细胞系表现出统计学显著（***P < 0.0001）的抗癌活性，而豆蔻油也对皮肤癌细胞系表现出统计学显著（***P < 0.0001）的抗癌活性，但仅对皮肤癌细胞系表现出活性。
