BMC Complement Altern Med. 2013 Jan 29;13:24. doi: 10.1186/1472-6882-13-24.
A number of bio-active secondary metabolites have been identified and reported for several Hypericum species. Many studies have reported the potential use of the plant extracts against several pathogens. However, Hypericum triquetrifolium is one of the least studied species for its antimicrobial activity. The aim of the present study was to evaluate the cytotoxic effect of the essential oils of Hypericum triquetrifolium as well as their antimicrobial potential against coxsakievirus B3 and a range of bacterial and fungal strains.
The essential oils of Hypericum triquetrifolium harvested from five different Tunisian localities (Fondouk DJedid, Bou Arada, Bahra, Fernana and Dhrea Ben Jouder) were evaluated for their antimicrobial activities by micro-broth dilution methods against bacterial and fungal strains. In addition, the cytotoxic effect and the antiviral activity of these oils were carried out using Vero cell lines and coxsakievirus B3.
The results showed a good antibacterial activities against a wide range of bacterial strains, MIC values ranging between 0.39-12.50 mg/ml and MBC values between 1.56-25.0 mg/ml. In addition, the essential oils showed promising antifungal activity with MIC values ranging between 0.39 μg/mL and 12.50 μg/mL; MFC values ranged between 3.12 μg/mL and 25.00 μg/mL; a significant anticandidal activity was noted (MIC values comprised between 0.39 μg/mL and 12.50 μg/mL). Although their low cytotoxic effect (CC50 ranged between 0.58 mg/mL and 12.00 mg/mL), the essential oils did not show antiviral activity against coxsakievirus B3.
The essential oils obtained from Hypericum triquetrifolium can be used as antimicrobial agents and could be safe at non cytotoxic doses. As shown for the tested essential oils, comparative analysis need to be undertaken to better characterize also the antimicrobial activities of Hypericum triquetrifolium extracts with different solvents as well as their purified fractions and their pure secondary metabolites.
已经鉴定和报道了多种贯叶金丝桃属植物的许多生物活性次生代谢产物。许多研究报告了植物提取物对几种病原体的潜在用途。然而，贯叶金丝桃是研究最少的物种之一，因为它的抗菌活性。本研究的目的是评估贯叶金丝桃的精油的细胞毒性作用及其对柯萨奇病毒 B3 和一系列细菌和真菌菌株的抗菌潜力。
用微量肉汤稀释法评价了从突尼斯五个不同地区（Fondouk DJedid、Bou Arada、Bahra、Fernana 和 Dhrea Ben Jouder）采集的贯叶金丝桃的精油的抗菌活性，对细菌和真菌菌株进行了评估。此外，还利用 Vero 细胞系和柯萨奇病毒 B3 进行了这些油的细胞毒性和抗病毒活性的检测。
结果表明，这些精油对广泛的细菌菌株具有良好的抗菌活性，MIC 值在 0.39-12.50mg/ml 之间，MBC 值在 1.56-25.0mg/ml 之间。此外，这些精油还表现出良好的抗真菌活性，MIC 值在 0.39μg/ml 和 12.50μg/ml 之间；MFC 值在 3.12μg/ml 和 25.00μg/ml 之间；对念珠菌有显著的抗活性（MIC 值在 0.39μg/ml 和 12.50μg/ml 之间）。尽管它们的细胞毒性低（CC50 值在 0.58mg/ml 和 12.00mg/ml 之间），但这些精油对柯萨奇病毒 B3 没有抗病毒活性。
贯叶金丝桃精油可作为抗菌剂使用，在非细胞毒性剂量下可能是安全的。正如测试的精油所显示的，需要进行比较分析，以更好地描述贯叶金丝桃提取物的抗菌活性，以及不同溶剂的提取物、其纯化部分和它们的纯次生代谢物的抗菌活性。
