Mousavi Seyed Ali, Hermundstad Brita, Flesland Annika Kristina, Llohn Abid Hussain, Saether Per Christian
Department of Immunology and Transfusion Medicine, Akershus University Hospital, Lørenskog, Norway.
Department of Multidisciplinary Laboratory Medicine and Medical Biochemistry, Akershus University Hospital, Lørenskog, Norway.
J Blood Med. 2022 Jun 3;13:283-292. doi: 10.2147/JBM.S362461. eCollection 2022.
Whilst there has been investigation into the effect of time of the day on platelet activation and function in healthy individuals, there is a lack of studies in the literature to examine this relationship among platelet donors.
We assessed the extent of platelet activation by percentage of platelets with surface-expressed P-selectin and flow cytometry in samples of whole blood from a group of qualified platelet donors (n = 84).
The mean (SD) percentage of activated platelets in the pre-donation blood samples was 1.85 ± 1.57% (range 0.2-7.5%). In univariate analyses, the percentage of activated platelets was significantly and inversely correlated with the collection time (ie, the time of day blood samples were collected) (r = -0.35, p = 0.001) and positively correlated to mean platelet volume (MPV) (r = 0.29, p = 0.008). A weaker positive correlation was also observed with ABO blood group (r = 0.228, p = 0.036). Analysis of the collection time as a categorical variable showed a greater degree of activated platelets in samples collected between 8:00 h and 10:00 h than in samples collected during the hours of >10:00 h ≤14:00 h (2.5 ± 1.8 versus 1.1 ± 0.74, p < 0.001). In the adjusted linear regression model, collection time was a significant independent predictor of platelet activation state in whole blood (β = -0.26; p < 0.001), as did ABO blood group (β = 0.55; p = 0.019).
Our results show that collection time is the most important predictor of platelet activation state in pre-donation whole blood among platelet donors. This work may have implications for optimizing the timing of platelet donation.
虽然已有研究探讨一天中的不同时间对健康个体血小板活化及功能的影响，但文献中缺乏关于血小板捐献者中这种关系的研究。
我们通过流式细胞术检测一组合格血小板捐献者（n = 84）全血样本中表面表达P-选择素的血小板百分比，以评估血小板活化程度。
献血前血样中活化血小板的平均（标准差）百分比为1.85±1.57%（范围0.2 - 7.5%）。在单因素分析中，活化血小板百分比与采集时间（即采集血样的时间）显著负相关（r = -0.35，p = 0.001），与平均血小板体积（MPV）正相关（r = 0.29，p = 0.008）。与ABO血型也观察到较弱的正相关（r = 0.228，p = 0.036）。将采集时间作为分类变量分析显示，8:00至10:00采集的样本中活化血小板程度高于10:00至14:00采集的样本（2.5±1.8对1.1±0.74，p < 0.001）。在调整后的线性回归模型中，采集时间是全血中血小板活化状态的显著独立预测因素（β = -0.26；p < 0.001），ABO血型也是（β = 0.55；p = 0.019）。
我们的结果表明，采集时间是血小板捐献者献血前全血中血小板活化状态的最重要预测因素。这项工作可能对优化血小板捐献时间具有启示意义。
