Boonyayothin Wanuttha, Kobtrakul Khwanlada, Khositanon Petlada, Vimolmangkang Sornkanok, Phoolcharoen Waranyoo
Center of Excellence in Plant-Produced Pharmaceuticals, Chulalongkorn University, Bangkok, Thailand.
Pharmaceutical Sciences and Technology Program, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Biotechnol Rep (Amst). 2022 Mar 26;34:e00725. doi: 10.1016/j.btre.2022.e00725. eCollection 2022 Jun.
Δ-9-tetrahydrocannabinol (Δ9-THC) is mainly a psychoactive compound in the cannabis plant. The immunoassay, an alternative method to HPLC and GC, can be used to analyze and measure Δ9-THC. This method provides high sensitivity and specificity by using antibodies specific to the desired substances. Currently, plants provide several benefits over traditional expression platforms to produce recombinant antibodies, such as lower production costs and scalability. Therefore, this study aims to produce a recombinant anti-Δ9-THC monoclonal antibody (mAb) with transient expression using . The highest expression level of the plant-produced mAb was estimated to be 0.33 ug/g leaf fresh weight. Our results demonstrate that the antibody provided in vitro affinity binding related to Δ9-THC and the metabolites of Δ9-THC, such as cannabinol (CBN). Moreover, the antibody also showed binding efficiency with Δ9-THC in cannabis extract. Moreover, plant-produced mAbs provide efficiency against Δ9-THC and can be applied for further immunoassay applications.
Δ-9-四氢大麻酚（Δ9-THC）是大麻植物中的主要精神活性化合物。免疫测定法作为高效液相色谱法（HPLC）和气相色谱法（GC）的替代方法，可用于分析和测量Δ9-THC。该方法通过使用针对所需物质的特异性抗体提供高灵敏度和特异性。目前，与传统表达平台相比，植物在生产重组抗体方面具有多种优势，例如生产成本更低且可扩展性强。因此，本研究旨在利用[具体方法]通过瞬时表达生产重组抗Δ9-THC单克隆抗体（mAb）。植物产生的mAb的最高表达水平估计为0.33微克/克叶片鲜重。我们的结果表明，该抗体在体外与Δ9-THC及其代谢物如大麻酚（CBN）具有亲和结合。此外，该抗体在大麻提取物中也显示出与Δ9-THC的结合效率。此外，植物产生的mAb对Δ9-THC具有效力，可用于进一步的免疫测定应用。
