Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av IPN 2508 Col Zacatenco, CDMX, 07360, Ciudad de México, Mexico.
Computer Science Department, University of Nebraska-Lincoln (UNL), Lincoln, NE, USA.
Arch Microbiol. 2022 Jun 10;204(7):382. doi: 10.1007/s00203-022-03009-2.
Horses are non-ruminant, herbivorous mammals, been used through history for various purposes, with a gut microbiota from cecum to the colon, possessing remarkable fermentative capacity. We studied the fecal microbiota of Azteca, Criollo, Frisian, Iberian, Pinto, Quarter and Spanish horse breeds living in Mexico by next-generation DNA sequencing of 16S rRNA gene libraries. Dominant phyla Firmicutes, Bacteroidetes, Proteobacteria, Spirochaetes, Fibrobacteres, Actinobacteria and Verrucomicrobia have different relative abundances among breeds, with contrasted alpha and beta diversities as well. Heatmap analysis revealed that Ruminococcaceae, Lachnospiraceae, Mogibacteriaceae families, and order Clostridiales are more abundant in Spanish, Azteca, Quarter and Criollo breeds. The LEfSe analysis displayed higher abundance of order Bacteroidales, family BS11, and genera Faecalibacterium, Comamonas, Collinsella, Acetobacter, and Treponema in Criollo, Azteca, Iberian, Spanish, Frisian, Pinto, and Quarter horse breeds. The conclusion is that dominant bacterial taxa, found in fecal samples of horse breeds living in Mexico, have different relative abundances.
马是非反刍草食性哺乳动物，历史上被用于各种用途，其肠道微生物群从盲肠延伸到结肠，具有显著的发酵能力。我们通过对生活在墨西哥的阿兹特克马、克里奥罗马、弗里西亚马、伊比利亚马、品脱马、夸特马和西班牙马等 6 个品种的粪便微生物组进行下一代 DNA 测序 16S rRNA 基因文库，研究了它们的粪便微生物群。厚壁菌门、拟杆菌门、变形菌门、螺旋体门、纤维杆菌门、放线菌门和疣微菌门是主要的菌门，在不同品种之间相对丰度不同，其α多样性和β多样性也有差异。热图分析显示，西班牙马、阿兹特克马、夸特马和克里奥罗马品种中，瘤胃球菌科、lachnospiraceae 科和 mogibacteriaceae 科以及梭状芽胞杆菌目更为丰富。LEfSe 分析显示，在克里奥罗马、阿兹特克马、伊比利亚马、西班牙马、弗里西亚马、品脱马和夸特马品种中，拟杆菌目、BS11 科和粪杆菌属、粪球菌属、柯林斯氏菌属、醋酸杆菌属和密螺旋体属的丰度更高。总之，在生活在墨西哥的马品种的粪便样本中发现的优势细菌类群存在不同的相对丰度。
