具有增强模拟过氧化物酶性能的牡丹状 3D-MoS/graphene 纳米结构用于多巴胺的比色测定。

Peony-like 3D-MoS/graphene nanostructures with enhanced mimic peroxidase performance for colorimetric determination of dopamine.

机构信息

Institute of Biological and Medical Engineering, Guangdong Academy of Sciences & National Engineering Research Center for Healthcare Devices, Guangzhou, Guangdong, 510500, China; Guangdong Engineering Technology Research Center for Diagnosis and Rehabilitation of Dementia, Guangzhou 510500, China.

出版信息

Talanta. 2022 Sep 1;247:123553. doi: 10.1016/j.talanta.2022.123553. Epub 2022 May 22.

DOI:10.1016/j.talanta.2022.123553

PMID:35688100

Abstract

Fast and quantitative detection of dopamine (DA) is highly desirable but still challenging in the clinical diagnosis due to the complexity of the biological samples. Herein, a novel peony-like 3D-MoS/Graphene (peony-like 3D-MoS/GR) nanomaterial is designed and characterized. The nanomaterial exhibits outstanding the peroxidase-like activity, which can be employed as a nanozyme for facile and fast colorimetric determination of dopamine. The peony-like 3D-MoS/GR-based colorimetric assay presents a wide liner dependence on the DA concentration in the range of 1-400 μM and a low detection limit of 0.21 μM. Such excellent results originate from its unique structure, which offers abundant active sites, high specific surface area, interconnected network and the special microenvironment. Furthermore, this reported colorimetric assay is successfully employed for detection of DA in human serum and urine samples. The recovery percentage ranges from 98 to 105.6%. Hence, the peony-like 3D-MoS/GR paves a new avenue for detection of DA in real biological systems.

摘要

由于生物样本的复杂性，快速、定量检测多巴胺（DA）在临床诊断中非常重要，但仍然具有挑战性。在此，设计并表征了一种新型的牡丹状 3D-MoS/石墨烯（peony-like 3D-MoS/GR）纳米材料。该纳米材料表现出优异的过氧化物酶样活性，可作为纳米酶用于多巴胺的简便、快速比色测定。基于牡丹状 3D-MoS/GR 的比色分析在 1-400 μM 的范围内对 DA 浓度表现出宽线性依赖性，检测限低至 0.21 μM。如此优异的结果源于其独特的结构，提供了丰富的活性位点、高比表面积、互联网络和特殊的微环境。此外，该报道的比色分析方法成功地用于检测人血清和尿液样本中的 DA。回收率在 98%至 105.6%之间。因此，牡丹状 3D-MoS/GR 为在真实生物体系中检测 DA 开辟了新途径。