Manzione N C, Bagchi S, Das K M
Dig Dis Sci. 1987 Jun;32(6):593-7. doi: 10.1007/BF01296158.
Theories on the etiology of Crohn's disease have included extrinsic agents and intrinsic bowel wall defects. We sought to determine the presence of immunoreactive antigens specific to Crohn's disease tissue by modifying the enzyme-linked immunosorbent assay. Tissue proteins were extracted from four patients with Crohn's disease and from four normal segments of colon from patients with colonic cancer. These tissue extracts were further purified on Con A Sepharose 4B affinity column. The glycoproteins eluted from this column were adsorbed by polystyrene plates as antigen and tested against 85 sera from patients with Crohn's disease, ulcerative colitis, other diarrheal diseases, and normal subjects. Sera from 48 patients with Crohn's disease showed significantly greater recognition of Crohn's disease tissue glycoproteins than sera from 27 disease controls (P less than 0.0125) and 10 normal subjects. These Crohn's disease sera also showed preferential recognition of glycoproteins extracted from Crohn's disease tissue compared to glycoproteins from normal colonic tissue (P less than 0.0005). The nature of these immunoreactive proteins, whether extrinsic or intrinsic, is not yet known. The ELISA may help in further characterization of Crohn's disease tissue-specific glycoprotein(s) and to develop a clinically useful serological test.
关于克罗恩病病因的理论包括外部因素和肠壁内在缺陷。我们试图通过改进酶联免疫吸附测定法来确定克罗恩病组织特有的免疫反应性抗原的存在。从4例克罗恩病患者以及4例结肠癌患者的正常结肠段提取组织蛋白。这些组织提取物在伴刀豆球蛋白A琼脂糖4B亲和柱上进一步纯化。从该柱上洗脱的糖蛋白作为抗原吸附于聚苯乙烯板上，并针对85份来自克罗恩病患者、溃疡性结肠炎患者、其他腹泻病患者及正常受试者的血清进行检测。48例克罗恩病患者的血清对克罗恩病组织糖蛋白的识别显著高于27例疾病对照患者（P<0.0125）及10例正常受试者的血清。与正常结肠组织的糖蛋白相比，这些克罗恩病血清对从克罗恩病组织中提取的糖蛋白也表现出优先识别（P<0.0005）。这些免疫反应性蛋白是外源性还是内源性的，其性质尚不清楚。酶联免疫吸附测定法可能有助于进一步鉴定克罗恩病组织特异性糖蛋白，并开发出具有临床应用价值的血清学检测方法。
