Department of Anesthesiology, Jing'an District Central Hospital, Shanghai, China.
J Clin Lab Anal. 2022 Jul;36(7):e24542. doi: 10.1002/jcla.24542. Epub 2022 Jun 11.
T-helper (Th) cells regulate immunity and inflammation, and modulate cognitive impairment in both cardio-cerebrovascular and neurological diseases. This study aimed to explore the correlation of longitudinal change of Th1/2/17 cells with cognitive impairment and prognosis in acute ischemic stroke (AIS).
Th1/2/17 cells were detected by flow cytometry in peripheral blood samples from 150 AIS patients at admission (baseline), Day (D)1, D3, and D7 after admission, and from 30 controls. Mini-Mental State Examination (MMSE) score among AIS patients at discharge was assessed. Stroke recurrence and mortality were evaluated.
Th1 (p = 0.013) and Th17 cells (p < 0.001) but not Th2 cells (p = 0.105) were elevated in AIS patients versus controls. Th1 cells (p = 0.027) and Th17 cells (p < 0.001) but not Th2 cells (p = 0.227) were positively correlated with NIHSS score in AIS patients. Furthermore, Th1 and Th17 cells elevated from baseline to D3 and then decreased on D7 after AIS onset, while Th2 cells illustrated an opposite trend (all p < 0.001). Th17 cells on D1 (p = 0.011), D3 (p = 0.014), and D7 (p < 0.001) were correlated with lower MMSE score, and their levels on D3 (p = 0.033) and D7 (p = 0.004) were related to elevated cognitive impairment. Th1 and Th2 cells were not related to cognitive function (all p > 0.05). Additionally, Th17 cells at baseline, D1, D3, and D7 (all p < 0.05) were increased in recurrent patients versus non-recurrent patients, and in survived patients versus dead patients, but Th1 or Th2 cells did not vary (all p > 0.05).
Th17 cells correlate with increased cognitive impairment, stroke recurrence, and mortality among AIS patients.
辅助性 T 细胞（Th）调节免疫和炎症，并调节心脑血管和神经疾病中的认知障碍。本研究旨在探讨 Th1/2/17 细胞的纵向变化与急性缺血性中风（AIS）患者认知障碍和预后的相关性。
采用流式细胞术检测 150 例 AIS 患者入院时（基线）、入院后第 1 天（D1）、第 3 天（D3）和第 7 天（D7）及 30 例对照者外周血中的 Th1/2/17 细胞。评估 AIS 患者出院时的简易精神状态检查（MMSE）评分。评估卒中复发和死亡率。
与对照组相比，AIS 患者的 Th1（p=0.013）和 Th17 细胞（p<0.001）升高，但 Th2 细胞（p=0.105）没有升高。AIS 患者的 Th1 细胞（p=0.027）和 Th17 细胞（p<0.001）与 NIHSS 评分呈正相关，但 Th2 细胞（p=0.227）没有相关性。此外，Th1 和 Th17 细胞从基线升高到 AIS 发病后第 3 天，然后在第 7 天下降，而 Th2 细胞则呈现相反的趋势（均 p<0.001）。D1（p=0.011）、D3（p=0.014）和 D7（p<0.001）的 Th17 细胞与较低的 MMSE 评分相关，D3（p=0.033）和 D7（p=0.004）的 Th17 细胞与认知障碍升高有关。Th1 和 Th2 细胞与认知功能无关（均 p>0.05）。此外，复发患者与未复发患者、存活患者与死亡患者相比，基线、D1、D3 和 D7 的 Th17 细胞均升高（均 p<0.05），而 Th1 或 Th2 细胞无差异（均 p>0.05）。
Th17 细胞与 AIS 患者认知障碍加重、卒中复发和死亡相关。
