Department of Geriatrics, HanDan Central Hospital, Handan, China.
Department of Rehabilitation Medicine, The First Hospital of Shanxi Medical University, Taiyuan, China.
J Clin Lab Anal. 2022 Oct;36(10):e24607. doi: 10.1002/jcla.24607. Epub 2022 Sep 4.
Acetyl-coenzyme A carboxylase 1 (ACC1) regulates lipid homeostasis, T helper (Th) cell differentiation, oxidative stress, inflammation response, and neurological process, engaging in acute ischemic stroke (AIS) pathogenesis, while its clinical utility in AIS is unclear. Hence, this study intended to explore the correlation among blood ACC1, Th17, and Th1 cells, and ACC1's potency as a prognostic biomarker for AIS management.
ACC1 in peripheral blood mononuclear cells (PBMCs) of 160 AIS patients and 30 controls were determined using RT-qPCR; blood Th17 and Th1 cells in AIS patients were quantified by flow cytometry.
ACC1 was increased in AIS patients compared with controls (median (interquartile range): 2.540 (1.753-3.548) vs. 0.980 (0.655-1.743), p < 0.001), which exhibited a good value to reflect AIS risk with the area under the curve of 0.872 (95% CI: 0.805-0.939). Moreover, ACC1 was positively linked with Th17 (r = 0.374, p < 0.001) and Th1 (r = 0.178, p = 0.024) cells in AIS patients. Additionally, ACC1 (r = 0.328, p < 0.001), Th17 (r = 0.272, p = 0.001), and Th1 cells (r = 0.195, p = 0.014) were positively associated with the National Institutes of Health Stroke Scale score in AIS patients. ACC1 high vs. low (p = 0.038) and Th17 high vs. low (p = 0.026) were related to shortened recurrence-free survival (RFS) in AIS patients, while Th1 cells (p = 0.179) were not correlated with RFS. Whereas ACC1 (p = 0.248), Th17 (p = 0.079), and Th1 cells (p = 0.130) were not linked with overall survival (OS) in AIS patients.
Circulating ACC1 overexpression correlates with increased Th17, Th1 cells, NIHSS score, and shortened RFS in AIS patients.
乙酰辅酶 A 羧化酶 1（ACC1）调节脂质稳态、辅助性 T 细胞（Th）分化、氧化应激、炎症反应和神经过程，参与急性缺血性脑卒中（AIS）的发病机制，但其在 AIS 中的临床应用尚不清楚。因此，本研究旨在探讨外周血 ACC1、Th17 和 Th1 细胞之间的相关性，以及 ACC1 作为 AIS 管理的预后生物标志物的潜力。
采用 RT-qPCR 检测 160 例 AIS 患者和 30 例对照者外周血单个核细胞（PBMCs）中的 ACC1；采用流式细胞术检测 AIS 患者血中 Th17 和 Th1 细胞。
与对照组相比，AIS 患者的 ACC1 升高（中位数（四分位距）：2.540（1.753-3.548）vs. 0.980（0.655-1.743），p<0.001），其曲线下面积为 0.872（95%CI：0.805-0.939），具有良好的反映 AIS 风险的价值。此外，ACC1 与 AIS 患者的 Th17（r=0.374，p<0.001）和 Th1（r=0.178，p=0.024）细胞呈正相关。此外，ACC1（r=0.328，p<0.001）、Th17（r=0.272，p=0.001）和 Th1 细胞（r=0.195，p=0.014）与 AIS 患者的国立卫生研究院卒中量表评分呈正相关。ACC1 高 vs. 低（p=0.038）和 Th17 高 vs. 低（p=0.026）与 AIS 患者的复发无事件生存（RFS）缩短相关，而 Th1 细胞（p=0.179）与 RFS 无关。而 ACC1（p=0.248）、Th17（p=0.079）和 Th1 细胞（p=0.130）与 AIS 患者的总生存（OS）无关。
循环 ACC1 过度表达与 AIS 患者 Th17、Th1 细胞、NIHSS 评分升高和 RFS 缩短相关。
