Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, and Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural Products, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.
Key Laboratory of Ministry of Education for Medicinal Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, China.
J Sci Food Agric. 2022 Dec;102(15):7039-7051. doi: 10.1002/jsfa.12065. Epub 2022 Jul 6.
Fuzhuan brick tea (FBT) has been shown to prevent obesity, but little is known about the effect of Eurotium cristatum, a critical fungus from FBT. This study examined the effects of live E. cristatum on lipid metabolism and gut microbiota composition in high-fat (HF) diet-induced obese mice.
Male HF diet-fed mice were treated with E. cristatum for 12 weeks. The results showed that E. cristatum administration caused strong inhibition against HF-induced body weight gain, dyslipidemia and liver oxidative stress damage. Additionally, Firmicutes and Bacteroidetes in phylum level and six types of bacterial including short-chain fatty acids (SCFAs) producing bacteria in genus level were found to be significantly changed in E. cristatum treated mice as compared to HF fed mice. As expected, E. cristatum could increase total SCFAs levels in feces. Interestingly, E. cristatum markedly increased the proportion of Akkermansia to resist obesity. Functional prediction analysis indicated that E. cristatum changed lipid and energy metabolism. Furthermore, E. cristatum ingestion can modulate hepatic acetyl-coa carboxylase (ACC), fatty acid synthase (FAS), sterol-regulatory element binding protein-1 (SREBP-1) and adipose uncoupling protein-1 (UCP-1) expression.
Conclusively, these findings suggest that E. cristatum can prevent the HF-induced lipid accumulation and other complications by modulating gut microbiota, lipid and energy metabolism. © 2022 Society of Chemical Industry.
茯砖茶（FBT）已被证明可预防肥胖，但对于 FBT 中的关键真菌——纯绿青霉（Eurotium cristatum），其对肥胖的影响知之甚少。本研究探讨了活纯绿青霉对高脂（HF）饮食诱导肥胖小鼠脂代谢和肠道微生物群落组成的影响。
雄性 HF 饮食喂养的小鼠用纯绿青霉处理 12 周。结果表明，纯绿青霉给药对 HF 诱导的体重增加、血脂异常和肝氧化应激损伤具有强烈的抑制作用。此外，与 HF 喂养的小鼠相比，在门水平上厚壁菌门和拟杆菌门以及属水平上的六种包括产生短链脂肪酸（SCFAs）的细菌在内的细菌类型明显发生变化。如预期的那样，纯绿青霉可增加粪便中总 SCFAs 水平。有趣的是，纯绿青霉可显著增加 Akkermansia 的比例以抵抗肥胖。功能预测分析表明，纯绿青霉改变了脂质和能量代谢。此外，纯绿青霉摄入可调节肝乙酰辅酶 A 羧化酶（ACC）、脂肪酸合酶（FAS）、固醇调节元件结合蛋白-1（SREBP-1）和脂肪解偶联蛋白-1（UCP-1）的表达。
总之，这些发现表明，纯绿青霉通过调节肠道微生物群、脂质和能量代谢来预防 HF 诱导的脂质积累和其他并发症。 © 2022 化学工业协会。
