Chen Si-Kai, Wei Wen-Xin, Huang Feng-Yu, Wang Jing, Li Xing-Yu, Yang Yu-Ting, Xing Wan-Tao, Gao Feng, Li Min, Miao Feng, Chen Liang-Liang, Wei Pei-Feng
Shaanxi University of Chinese Medicine, Xianyang, 712046, China.
Nanchang University, Nanchang, 330029, China.
Heliyon. 2024 Jun 4;10(12):e32343. doi: 10.1016/j.heliyon.2024.e32343. eCollection 2024 Jun 30.
Hyperlipidemia (HLP) presents a significant challenge to global public health. Mounting evidence suggests that statins, the recommended first-line lipid-lowering agents, have significant adverse effects. Consequently, the quest for natural and efficacious alternative therapies is steadily emerging as a research priority for HLP prevention and treatment. Consumption of tea, which is rich in diverse biologically active compounds with the capacity to regulate lipid metabolism and combat obesity, has emerged as a promising alternative therapy. Sea buckthorn leaves are rich in a multitude of biologically active substances, have a hypolipidemic effect, and can be used as a raw material for tea because of their unique flavor. There is a suggestion that combining with tea could modify or boost the lipid-lowering active compounds present in tea, thereby increasing its efficacy in regulating lipid metabolism.
Sea Buckthorn Leaf Fu Tea (SBLFT) was obtained by fermentation when sea buckthorn leaves contained 42 % moisture, inoculated with 0.2 mL/g, and incubated for 8 d at constant temperature. Animal experiments demonstrated that SBLFT significantly inhibited body weight gain in HLP rats and reduced lipid content and serum oxidative stress. In addition, liver tissue sections and functional indices showed that SBLFT can improve liver morphology and function abnormalities. Reverse transcription-polymerase chain reaction results indicated that the expression of Liver kinase B1 (), adenosine 5'-monophosphate (AMP)-activated protein kinase (), acetyl CoA carboxylase 1 (ACC1), and sterol-regulatory element binding protein-1 () gene related to lipid metabolism was altered.
SBLFT improved HLP, specifically via promoting the expression of in the liver of HLP rats, activating , and inhibiting and expression, resulting in the inhibition of fatty acid and triglyceride synthesis-related enzymes at the transcriptional level.
高脂血症（HLP）对全球公共卫生构成重大挑战。越来越多的证据表明，作为推荐的一线降脂药物，他汀类药物有显著的不良反应。因此，寻求天然且有效的替代疗法正逐渐成为HLP防治研究的重点。茶富含多种具有调节脂质代谢和对抗肥胖能力的生物活性化合物，已成为一种有前景的替代疗法。沙棘叶富含多种生物活性物质，具有降血脂作用，因其独特风味可作为茶的原料。有人认为与茶结合可以改变或增强茶中存在的降脂活性化合物，从而提高其调节脂质代谢的功效。
当沙棘叶含水量为42%，接种量为0.2 mL/g，并在恒温下培养8天时，通过发酵获得沙棘叶茯茶（SBLFT）。动物实验表明，SBLFT显著抑制HLP大鼠体重增加，降低脂质含量和血清氧化应激。此外，肝组织切片和功能指标显示，SBLFT可以改善肝脏形态和功能异常。逆转录-聚合酶链反应结果表明，与脂质代谢相关的肝激酶B1（）、5'-单磷酸腺苷（AMP）激活的蛋白激酶（）、乙酰辅酶A羧化酶1（ACC1）和固醇调节元件结合蛋白-1（）基因的表达发生了改变。
SBLFT改善了HLP，具体是通过促进HLP大鼠肝脏中 的表达，激活 ，并抑制 和 的表达，从而在转录水平上抑制脂肪酸和甘油三酯合成相关酶。
