Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Engineering Research Center of Chinese Medicine Resource, Ministry of Education, Beijing, China.
J Appl Microbiol. 2022 Sep;133(3):1555-1565. doi: 10.1111/jam.15662. Epub 2022 Jun 24.
Lycii Fructus (LF) is considered as a 'superfood' due to its health benefits and delicious tastes, which has gained popularity worldwide. However, LF is also a proper host for fungal growth due to its abundant nutrients. Fungal contamination seriously affects the quality and safety of LF and poses threats to consumer health.
In this study, a total of 15 LF samples were collected from five provinces in China, and were divided into five groups based on the collection areas. Fungal contamination in LF was investigated by targeting the internal transcribed spacer 2 region using Illumina Miseq PE300 platform, and the differences of fungal community in groups based on collection areas were compared. Results showed that the fungal contamination was detected in all the 15 LF samples. Ascomycota, Dothideomycetes, Pleosporales and Pleosporaceae were dominant at the phylum, class, order and family levels, respectively. At the genus level, Alternaria, Cladosporium and Fusarium were the three dominant genera. In all, 24 fungal species were identified. Among which, two species, namely Penicillium oxalicum and Trichothecium roseum, were potentially toxigenic.
All 15 LF samples were detected with fungal contamination. The differences of fungal community in LF samples collected from different areas were observed. DNA metabarcoding was demonstrated as an efficient method to monitor the fungal contamination in LF.
This work comprehensively reveals the fungal diversity and composition in LF and provides early warning for potential mycotoxin contamination.
由于具有健康益处和美味口感，枸杞（LF）被认为是一种“超级食品”，在全球范围内广受欢迎。然而，由于其丰富的营养成分，LF 也是真菌生长的合适宿主。真菌污染严重影响 LF 的质量和安全，对消费者健康构成威胁。
本研究共采集中国五个省份的 15 份 LF 样本，根据采集区域分为五组。通过 Illumina Miseq PE300 平台靶向内部转录间隔区 2 区调查 LF 中的真菌污染，并比较基于采集区域的组间真菌群落的差异。结果表明，所有 15 份 LF 样本均检测到真菌污染。子囊菌门、座囊菌纲、腔孢纲和腔孢科分别为优势菌门、纲、目和科。在属水平上，交替单胞菌属、枝孢属和镰刀菌属是三个优势属。共鉴定出 24 种真菌。其中，两株潜在产毒菌，即草酸青霉和玫瑰色镰刀菌。
所有 15 份 LF 样本均检测到真菌污染。来自不同地区的 LF 样本中的真菌群落存在差异。DNA 宏条形码被证明是监测 LF 中真菌污染的有效方法。
本研究全面揭示了 LF 中的真菌多样性和组成，为潜在的真菌毒素污染提供了早期预警。
