Yu Jingsheng, Guo Mengyue, Jiang Wenjun, Dao Yujie, Pang Xiaohui
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Front Nutr. 2022 May 12;9:883698. doi: 10.3389/fnut.2022.883698. eCollection 2022.
Crataegi Fructus, a medicinal and edible herb in China, has been considered a popular dietary supplement globally. It is used for the treatment of dyspepsia and chronic heart failure according to the Chinese Pharmacopoeia (2020). However, fungal contamination in Crataegi Fructus affects its quality and safety, thus preventing its global promotion. In this study, we comprehensively studied the fungal community in processed products of Crataegi Fructus by high-throughput sequencing. A total of 21 Crataegi Fructus samples were collected from five provinces in China, and the samples were divided into five groups based on collection areas, as well as into three groups based on processing methods. We then targeted the internal transcribed spacer 2 sequence through the Illumina Miseq PE300 platform to investigate fungal composition and diversity. Results showed that all 21 samples were detected with fungal contamination, and Ascomycota was dominant at the phylum level. In the groups based on collection areas, Dothideomycetes, Pleosporaceae, and were dominant at the class, family, and genus levels, respectively. In the groups based on processing methods, Dothideomycetes, Aspergillaceae, and were the most abundant at the class, family, and genus levels, respectively. Differences in fungal communities between various groups were also observed. Furthermore, a total of 115 species were identified, among which seven were potential toxigenic, namely, , , , , , , and . In conclusion, this study reveals great fungal richness and diversity of Crataegi Fructus, providing references for the prevention and control of fungal contamination of Crataegi Fructus in practical production.
山楂，一种在中国药食两用的草药，在全球范围内被视为一种受欢迎的膳食补充剂。根据《中国药典》（2020年版），它被用于治疗消化不良和慢性心力衰竭。然而，山楂中的真菌污染会影响其质量和安全性，从而阻碍其在全球的推广。在本研究中，我们通过高通量测序全面研究了山楂加工产品中的真菌群落。从中国五个省份共收集了21份山楂样品，并根据采集地区将样品分为五组，同时根据加工方法分为三组。然后，我们通过Illumina Miseq PE300平台靶向内部转录间隔区2序列，以研究真菌组成和多样性。结果表明，所有21个样品均检测到真菌污染，在门水平上子囊菌门占主导地位。在基于采集地区的组中，座囊菌纲、格孢腔菌科和分别在纲、科和属水平上占主导地位。在基于加工方法的组中，座囊菌纲、曲霉科和分别在纲、科和属水平上最为丰富。还观察到不同组之间真菌群落的差异。此外，共鉴定出115个物种，其中7个是潜在产毒真菌，即、、、、、和。总之，本研究揭示了山楂中丰富的真菌种类和多样性，为实际生产中山楂真菌污染的防控提供了参考。
