Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Int J Mol Sci. 2023 Oct 11;24(20):15081. doi: 10.3390/ijms242015081.
Lonicerae Japonicae Flos (LJF) has been globally applied as an herbal medicine and tea. A number of reports recently revealed fungal and mycotoxin contamination in medicinal herbs. It is essential to analyze the fungal community in LJF to provide an early warning for supervision. In this study, the fungal community in LJF samples was identified through DNA metabarcoding. A total of 18 LJF samples were collected and divided based on the collection areas and processing methods. The results indicated that Ascomycota was the dominant phylum. At the genus level, was the most abundant, followed by and . Ten pathogenic fungi were detected among the 41 identified species. Moreover, , , and had lower relative abundances in LJF samples under oven drying than under other processing methods. This work is expected to provide comprehensive knowledge of the fungal community in LJF and a theoretical reference for enhanced processing methods in practical manufacturing.
金银花被广泛用作草药和茶。最近有报道称，药用植物受到真菌和霉菌毒素的污染。因此，分析金银花中的真菌群落对于提供早期预警监管非常重要。本研究通过 DNA 代谢组学鉴定了金银花样品中的真菌群落。共采集了 18 个金银花样品，根据采集地区和加工方法进行了分组。结果表明，子囊菌门是优势菌门。在属水平上， 丰度最高，其次是 和 。在所鉴定的 41 个种中，检测到 10 种致病真菌。此外，与其他加工方法相比，在烘箱干燥条件下， 、 和 在金银花样品中的相对丰度较低。这项工作有望为金银花中的真菌群落提供全面的知识，并为实际生产中改进加工方法提供理论参考。
