Masih Zahra, Hoghooghirad Nasser, Madani Rasool, Sharbatkhori Mitra
Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
J Parasit Dis. 2022 Jun;46(2):377-383. doi: 10.1007/s12639-021-01454-2. Epub 2021 Sep 29.
Cystic Echinococcosis/Hydatidosis considered as one of the most important parasitic diseases in humans and animals across the world. The goal of the present study was to determine a native antigen with an acceptable sensitivity and specificity to be used in the human hydatid cyst diagnostic methods. In the present study, recombinant P29 antigen was used to detect the antibodies in the serum of patients with hydatid cysts of the liver. In fact, purified recombinant P29 protein is used as an antigen in ELISA. In order to evaluate the recombinant P29 protein for diagnostic ELISA, 25 serums obtained from people harboring the hydatid cysts were tested. The result of the gene expression on a 12% SDS-PAGE gel showed a band with a length of 28 KD. Also, 28KDa band was observed through the reaction of recombinant P29 protein with Anti T7-tag monoclonal antibody in the western blotting method. This protein showed satisfactory results in detecting the hydatid cyst antibodies in the serum of patients having hydatid cysts. Twenty two of 25 hydatidosis serums positively reacted in ELISA using with P29 protein, indicating in 92% of ELISA sensitivity, 95% of specificity, 95.83% of positive predictive value, and 90.42% of negative predictive value for recombinant P29 protein. Whereas the produced recombinant protein P29 showed promising results in the diagnosis of hydatidosis but of more research needs to be done to reach a more accurate conclusion.
囊型棘球蚴病/包虫病被认为是全球人类和动物最重要的寄生虫病之一。本研究的目的是确定一种具有可接受的敏感性和特异性的天然抗原，用于人类包虫囊肿的诊断方法。在本研究中，重组P29抗原用于检测肝包虫囊肿患者血清中的抗体。实际上，纯化的重组P29蛋白在酶联免疫吸附测定（ELISA）中用作抗原。为了评估用于诊断ELISA的重组P29蛋白，对从患有包虫囊肿的人身上获得的25份血清进行了检测。在12%的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上的基因表达结果显示出一条长度为28千道尔顿（KD）的条带。此外，在蛋白质印迹法中，通过重组P29蛋白与抗T7标签单克隆抗体的反应也观察到了28KDa的条带。这种蛋白在检测患有包虫囊肿患者血清中的包虫囊肿抗体方面显示出令人满意的结果。25份包虫病血清中有22份在使用P29蛋白的ELISA中呈阳性反应，表明重组P29蛋白的ELISA敏感性为92%，特异性为95%，阳性预测值为95.83%，阴性预测值为90.42%。虽然所产生的重组蛋白P29在包虫病诊断中显示出有前景的结果，但还需要进行更多研究以得出更准确的结论。
