Kartika Andy Visi, Iizasa Hisashi, Ding Dan, Kanehiro Yuichi, Tajima Yoshitsugu, Kaji Shunsuke, Yanai Hideo, Yoshiyama Hironori
Department of Microbiology, Faculty of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane 693-8504, Japan.
Department of Pathology Anatomy, Faculty of Medicine, University of Muslim Indonesia, Jl. Urip Sumoharjo KM.5, Makassar, Sulawesi 90231, Indonesia.
Microorganisms. 2020 Jun 18;8(6):923. doi: 10.3390/microorganisms8060923.
Persistent gastric mucosal damage caused by infection is a major risk factor for gastric cancer (GC). The Epstein-Barr virus (EBV) is also associated with GC. Most patients with EBV-associated GC are infected with in East Asia. However, very few reports have described where and when both and EBV infect the gastric mucosa. To clarify this, old biopsy samples used for the rapid urease test (RUT) were applied to count EBV genomic DNA (gDNA) copies using DNA probe quantitative polymerase chain reaction. DNA extracted from the gastric biopsy samples of 58 patients with atrophic gastritis was used to analyze the correlation between the degree of atrophic gastritis and the copy number of EBV gDNA. EBV was detected in 44 cases (75.9%), with viral copy numbers ranging from 12.6 to 4754.6. A significant correlation was found between patients with more than 900 copies of EBV gDNA and those with a more severe grade of atrophic gastritis ( = 0.041). This study shows that EBV can be detected in RUT samples in a manner that reduces patient burden.
感染引起的持续性胃黏膜损伤是胃癌（GC）的主要危险因素。爱泼斯坦-巴尔病毒（EBV）也与GC有关。大多数EBV相关GC患者在东亚地区感染。然而，很少有报告描述幽门螺杆菌和EBV在何时何地感染胃黏膜。为了阐明这一点，将用于快速尿素酶试验（RUT）的旧活检样本应用于使用DNA探针定量聚合酶链反应来计数EBV基因组DNA（gDNA）拷贝数。从58例萎缩性胃炎患者的胃活检样本中提取的DNA用于分析萎缩性胃炎程度与EBV gDNA拷贝数之间的相关性。在44例（75.9%）中检测到EBV，病毒拷贝数范围为12.6至4754.6。在EBV gDNA拷贝数超过900的患者与萎缩性胃炎更严重分级的患者之间发现了显著相关性（P = 0.041）。这项研究表明，可以以减轻患者负担的方式在RUT样本中检测到EBV。
