Department of Pathology, Central South University, Xiangya School of Medicine, Affiliated Haikou Hospital, Haikou, Hainan 570208, P.R. China.
Mol Med Rep. 2019 Mar;19(3):2125-2136. doi: 10.3892/mmr.2019.9886. Epub 2019 Jan 22.
AT‑rich interaction domain 1A (ARID1A) and phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit α (PIK3CA) serve important roles in the formation and development of numerous malignancies including gastric cancer. Accumulating evidence has demonstrated that Epstein‑Barr virus (EBV) is a pathogenic virus associated with gastric cancer. The present study aimed to investigate the association between EBV infection, and the expression levels of ARID1A and PIK3CA in gastric cancer. EBER in situ hybridization was performed to detect EBV infection. Immunohistochemistry was used to assess the expression levels of ARID1A and PIK3CA in gastric cancer and adjacent normal tissues. A total of 58 gastric cancer and 10 adjacent normal tissues were tested for genetic mutations via single nucleotide polymorphism genotyping assays. Fluorescent polymerase chain reaction was used to detect EBV infection; 9.3% (28/300) of gastric cancer samples were positive for EBV, whereas, all adjacent normal tissues were negative. ARID1A and PIK3CA were negatively correlated in gastric cancer (r=‑0.167). The expression levels of ARID1A and PIK3CA in gastric cancer were significantly associated with the depth of invasion of gastric cancer. A total of 62.1% (36/58) of tumor samples exhibited mutations in ARID1A, whereas, 13.8% (8/58) presented mutations in PIK3CA. Notably, EBV‑associated gastric cancer (EBVaGC) samples with PIK3CA mutations additionally exhibited ARID1A mutations. Although in the present study it was identified that ARID1A and PIK3CA were negatively correlated in EBVaGC, further studies are required to investigate the association among ARID1A, PIK3CA and EBV in gastric cancer.
富含 AT 序列结合域蛋白 1A（ARID1A）和磷脂酰肌醇-4,5-二磷酸 3-激酶催化亚单位α（PIK3CA）在包括胃癌在内的多种恶性肿瘤的形成和发展中发挥着重要作用。越来越多的证据表明，Epstein-Barr 病毒（EBV）是一种与胃癌相关的致病病毒。本研究旨在探讨 EBV 感染与胃癌中 ARID1A 和 PIK3CA 表达水平之间的关系。采用 EBER 原位杂交法检测 EBV 感染，免疫组织化学法检测胃癌及癌旁正常组织中 ARID1A 和 PIK3CA 的表达水平。采用单核苷酸多态性基因分型检测技术对 58 例胃癌和 10 例癌旁正常组织进行基因突检测。采用荧光聚合酶链反应检测 EBV 感染；300 例胃癌样本中，9.3%（28/300）呈 EBV 阳性，而所有癌旁正常组织均为阴性。胃癌中 ARID1A 和 PIK3CA 呈负相关（r=-0.167）。胃癌中 ARID1A 和 PIK3CA 的表达水平与胃癌浸润深度显著相关。58 例肿瘤样本中，62.1%（36/58）存在 ARID1A 突变，13.8%（8/58）存在 PIK3CA 突变。值得注意的是，伴有 PIK3CA 突变的 EBV 相关胃癌（EBVaGC）样本还存在 ARID1A 突变。尽管本研究表明 EBVaGC 中 ARID1A 和 PIK3CA 呈负相关，但仍需进一步研究以探讨 ARID1A、PIK3CA 和 EBV 之间在胃癌中的关系。
