Bartram C R, Berthold F
Eur J Pediatr. 1987 Mar;146(2):162-5. doi: 10.1007/BF02343225.
Genomic configuration and expression of the N-myc gene was investigated by Southern and Northern blot analyses in 18 neuroblastomas of different clinical stages. We observed a 4-100-fold amplification of this oncogene in one of six stage III, two of four stage IV as well as one of five stage IVS tumours. Remarkably, an 80-fold N-myc amplification was demonstrated in a patient with stage IV neuroblastoma being in remission for more than 2 years; moreover, a 100-fold amplification could be detected in a stage IVS tumour from a newborn. These data are discussed in view of the recently postulated close association of N-myc amplification with rapid progression of neuroblastomas.
通过Southern和Northern印迹分析，对18例不同临床分期的神经母细胞瘤进行了N-myc基因的基因组结构和表达研究。我们观察到，在6例III期肿瘤中的1例、4例IV期肿瘤中的2例以及5例IVS期肿瘤中的1例中，该癌基因有4至100倍的扩增。值得注意的是，在一名缓解超过2年的IV期神经母细胞瘤患者中发现了80倍的N-myc扩增；此外，在一名新生儿的IVS期肿瘤中检测到了100倍的扩增。鉴于最近推测的N-myc扩增与神经母细胞瘤快速进展的密切关联，对这些数据进行了讨论。
