School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian, Liaoning, China.
Academy of Food Interdisciplinary Science, Dalian Polytechnic University, Dalian, Liaoning, China.
PeerJ. 2022 Jun 7;10:e13513. doi: 10.7717/peerj.13513. eCollection 2022.
During electrospinning, the fibers deposited on the collector are usually randomly oriented in a disordered form. Researchers hope to generate periodic structures to expand the application of electrospinning, including improving the sensing properties of electronic and photonic devices, improving the mechanical properties of solid polymer composites and directional growth of human tissues. Here, we propose a technique to control the preparation of aligned foodborne nanofibers by placing dielectric polymers on microfluidic devices, which does not require the use of metal collectors. This study was conducted by introduced PEDOT:PSS polymer as a ground collector to prepare aligned foodborne nanofibers directly on the microfluidic platform. The fluidity of the electrolytic polymer collector makes it possible to shape the grounding collector according to the shape of the microcavity, thus forming a space adjustable nanofiber membrane with a controllable body. The simplicity of dismantling the collector also enables it extremely simple to obtain a complete electrospun fiber membrane without any additional steps. In addition, nanofibers can be easily stacked into a multi-layer structure with controllable hierarchical structures. The Caco-2 cells that grow on the device formed a compact intestinal epithelial layer that continuously expresses the tightly bound protein ZO-1. This intestinal barrier, which selectively filters small molecules, has a higher level of TEER, reproducing intestinal filtration functions similar to those of models. This method provides new opportunities for the design and manufacture of various tissue scaffolds, photonic and electronic sensors.
在静电纺丝过程中，沉积在收集器上的纤维通常以无序的形式随机定向。研究人员希望生成周期性结构以扩展静电纺丝的应用，包括提高电子和光子器件的传感性能、改善固体聚合物复合材料的机械性能以及促进人体组织的定向生长。在这里，我们提出了一种通过将介电聚合物放置在微流控器件上来控制取向食物源纳米纤维制备的技术，该技术不需要使用金属收集器。本研究通过引入 PEDOT：PSS 聚合物作为接地收集器，直接在微流控平台上制备取向的食物源纳米纤维。电解质聚合物收集器的流动性使得根据微腔的形状来塑造接地收集器成为可能，从而形成具有可调节空间的可调谐纳米纤维膜。收集器的简单拆卸也使得获得完整的无任何额外步骤的静电纺丝纤维膜变得极其简单。此外，纳米纤维可以很容易地堆叠成具有可控分层结构的多层结构。在该装置上生长的 Caco-2 细胞形成了紧密连接蛋白 ZO-1 表达紧密的完整肠上皮层。这种选择性过滤小分子的肠屏障具有更高的 TEER 值，复制了与模型类似的肠过滤功能。这种方法为各种组织支架、光子和电子传感器的设计和制造提供了新的机会。
