School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning, China.
National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian, Liaoning, China.
Crit Rev Food Sci Nutr. 2022;62(16):4418-4434. doi: 10.1080/10408398.2021.1875395. Epub 2021 Jan 22.
Microfluidic intestine-on-a-chip enables novel means of emulating human intestinal pathophysiology in vitro, which can potentially reduce animal testing and substitute simple 2D culture system. Though a great deal of work has been done in the development of microfluidic platforms for intestinal disease modeling and drug screening, potential investigation of the effect of bioactive food compounds on intestinal inflammation remains largely unexplored. In this review, different biomaterials and chip designs have been explored in the fabrication of intestine-on-a-chip. Other key parameters must be carefully controlled and selected, including shear stress, cell type and cell co-culture spatial configuration, etc. Appropriate techniques to quantify the barrier integrity including trans-epithelial electric resistance, specific tight junction markers and permeability measurements should be standardized and compared with in vivo data. Integration of the gut microbiome and the provision of intestinal-specific environment are the key parameters to realize the in vivo intestinal model simulation and accelerate the screening efficiency of bioactive food compounds.
微流控肠芯片为模拟人类肠道病理生理学提供了新方法，可潜在减少动物实验并替代简单的 2D 培养系统。尽管在开发用于肠道疾病建模和药物筛选的微流控平台方面已经开展了大量工作，但对生物活性食物化合物对肠道炎症影响的潜在研究仍在很大程度上尚未得到探索。在这篇综述中，研究人员探讨了不同的生物材料和芯片设计在肠芯片中的应用。其他关键参数也必须仔细控制和选择，包括剪切力、细胞类型和细胞共培养空间配置等。应标准化并与体内数据进行比较，以量化屏障完整性的适当技术，包括跨上皮电阻、特定紧密连接标记物和通透性测量。整合肠道微生物组和提供肠道特异性环境是实现体内肠道模型模拟和加速生物活性食物化合物筛选效率的关键参数。
