Department of Pathology, Bishan People's Hospital of Chongqing, Chongqing, 402760, People's Republic of China.
Department of Laboratory Medicine, Wanzhou People's Hospital of Chongqing, No. 27 Guoben Road, Wanzhou District, Chongqing, 404100, People's Republic of China.
J Cardiothorac Surg. 2021 Nov 8;16(1):327. doi: 10.1186/s13019-021-01701-w.
CircMMP11 is a characterized circRNA with oncogenic function in breast cancer. In this study, we explored the involvement of circMMP11 in non-small cell lung cancer (NSCLC).
Paired cancer and non-cancer tissues were collected from 66 NSCLC patients, and the expression of circMMP11 and miR-143 in these tissues were detected using RT-qPCRs. Overexpression levels of circMMP11 and miR-143 were performed by transfection, and their crosstalk was analyzed by RT-qPCRs. The effect of circMMP11 overexpression on miR-143 methylation was analyzed by methylation-specific PCR. CCK-8 assay was performed to analyze the roles of miR-143 and circMMP11 in regulating NSCLC cell proliferation.
We found that circMMP11 was overexpressed in NSCLC and predicted patients' poor survival. Moreover, a close correlation between circMMP11 and miR143 was observed. In NSCLC cells, circMMP11 overexpression reduced miR-143 expression and increased miR-143 methylation. CCK-8 assay analysis showed that miR-143 reversed the enhancing effects of circMMP11 overexpression on cell proliferation.
CircMMP11 is overexpressed in NSCLC and predicts poor survival. In addition, circMMP11 may downregulate miR-143 through methylation to suppress cell proliferation.
CircMMP11 是一种具有致癌功能的特征 circRNA，存在于乳腺癌中。在本研究中，我们探讨了 circMMP11 参与非小细胞肺癌（NSCLC）的情况。
从 66 例 NSCLC 患者中收集配对的癌组织和非癌组织，使用 RT-qPCR 检测这些组织中 circMMP11 和 miR-143 的表达。通过转染实现 circMMP11 和 miR-143 的过表达，并通过 RT-qPCR 分析它们的相互作用。通过甲基化特异性 PCR 分析 circMMP11 过表达对 miR-143 甲基化的影响。通过 CCK-8 分析检测 miR-143 和 circMMP11 在调节 NSCLC 细胞增殖中的作用。
我们发现 circMMP11 在 NSCLC 中过表达，并预测患者的生存不良。此外，还观察到 circMMP11 与 miR143 密切相关。在 NSCLC 细胞中，circMMP11 过表达降低了 miR-143 的表达并增加了 miR-143 的甲基化。CCK-8 分析表明，miR-143 逆转了 circMMP11 过表达对细胞增殖的增强作用。
CircMMP11 在 NSCLC 中过表达并预测生存不良。此外，circMMP11 可能通过甲基化下调 miR-143 来抑制细胞增殖。
