College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, Henan Province, PR China.
Int J Syst Evol Microbiol. 2022 Jun;72(6). doi: 10.1099/ijsem.0.005432.
A novel bacterium, designated NAS39, was isolated from the interfacial sediment of Taihu Lake in PR China and its taxonomic position was investigated by using a polyphasic approach. Cells of the isolate were Gram-stain-negative, aerobic, non-motile, catalase-positive, yellow and rod-shaped. Phylogenetic analyses based on 16S rRNA gene sequences supported that strain NAS39 formed a cluster within the genus , and was most closely related to LB2P30 (98.4 %), followed by 0563 (97.4 %). The average nucleotide identity values between strain NAS39 and LB2P30 and 0563 were 82.5 and 75.3 %, respectively. The digital DNA-DNA hybridization values between strain NAS39 and LB2P30 and 0563 were 40.9 and 18.6 %, respectively. The genomic DNA G+C content was 34.1 mol%. The major respiratory quinone was menaquinone-6. The dominant cellular fatty acids were iso-C and summed feature 3 comprising C ω7/C ω6. The polar lipids comprised phosphatidyl ethanolamine, two amino lipids, three amino phospholipids and two unidentified lipids. Based on the phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, strain NAS39 (=MCCC 1K06094=KACC 22328) represents a novel species of the genus , for which the name sp. nov. is proposed.
一种新型细菌，命名为 NAS39，从中国太湖界面沉积物中分离出来，并通过多相方法研究了其分类地位。该分离物的细胞革兰氏阴性、需氧、非运动、过氧化氢酶阳性、黄色和杆状。基于 16S rRNA 基因序列的系统发育分析表明，NAS39 菌株在属内形成一个聚类，与 LB2P30（98.4%）最为密切相关，其次是 0563（97.4%）。NAS39 菌株与 LB2P30 和 0563 的平均核苷酸同一性值分别为 82.5%和 75.3%。NAS39 菌株与 LB2P30 和 0563 的 DNA-DNA 杂交值分别为 40.9%和 18.6%。基因组 DNA G+C 含量为 34.1mol%。主要呼吸醌为menaquinone-6。主要细胞脂肪酸为 iso-C 和总和特征 3，包括 Cω7/Cω6。极性脂包括磷脂酰乙醇胺、两种氨基酸脂、三种氨基酸磷脂和两种未鉴定的脂。基于表型、化学分类、基因型和系统发育特征，NAS39 株（=MCCC 1K06094=KACC 22328）代表了属的一个新型种，提议将其命名为 sp. nov.。
