Yang Lei-Lei, Liu Qing, Liu Hong-Can, Zhou Yu-Guang, Xin Yu-Hua
1China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
2State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2019 Jun;69(6):1821-1825. doi: 10.1099/ijsem.0.003400. Epub 2019 Apr 17.
One bacterial strain, denoted as LB2P30, was isolated from Laigu glacier located on the Tibetan Plateau, PR China. Strain LB2P30 was an aerobic, non-motile, rod-shaped, yellow-pigmented and Gram-stain-negative bacterium. The temperature range for growth was 4-20 °C (optimum, 14 °C). The phylogenetic analysis based on partial 16S rRNA gene sequences showed that strain LB2P30 belonged to the genus Flavobacterium and was most similar to Flavobacterium fluvii (98.12 %) and Flavobacterium limicola (97.91 %). The average nucleotide identity values between strain LB2P30 and its closest relatives, F. fluvii DSM 19978 and F. limicola DSM 15094, were 76.73 % and 77.40 %, respectively. Cells of strain LB2P30 contained summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c) and iso-C15 : 0 as the predominant fatty acids and menaquinone-6 as the sole menaquinone. The genomic DNA G+C content was 34.74 mol%. Based on the phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, we propose strain LB2P30 as a novel species of the genus Flavobacterium with the nomenclature of Flavobacteriumlaiguense sp. nov. The type strain is LB2P30 (=CGMCC 1.11271=NBRC 113059).
从中国青藏高原的来古冰川中分离出一株细菌，命名为LB2P30。菌株LB2P30是一种需氧、无运动性、杆状、黄色色素沉着且革兰氏染色阴性的细菌。其生长温度范围为4-20°C（最适温度为14°C）。基于部分16S rRNA基因序列的系统发育分析表明，菌株LB2P30属于黄杆菌属，与河流黄杆菌（98.12%）和泥滩黄杆菌（97.91%）最为相似。菌株LB2P30与其最亲近的亲缘种河流黄杆菌DSM 19978和泥滩黄杆菌DSM 15094之间的平均核苷酸同一性值分别为76.73%和77.40%。菌株LB2P30的细胞含有特征性脂肪酸总和3（包含C16:1ω7c和/或C16:1ω6c）和异-C15:0作为主要脂肪酸，以及甲基萘醌-6作为唯一的甲基萘醌。基因组DNA的G+C含量为34.74 mol%。基于表型、化学分类、基因型和系统发育特征，我们提议将菌株LB2P30作为黄杆菌属的一个新物种，命名为来古黄杆菌（Flavobacterium laiguense sp. nov.）。模式菌株为LB2P30（=CGMCC 1.11271=NBRC 113059）。
