Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang, Jiangxi, PR China.
Int J Syst Evol Microbiol. 2024 Jun;74(6). doi: 10.1099/ijsem.0.006416.
A Gram-stain-negative, aerobic, non-spore-forming, nonmotile, rod-shaped, and yellow-pigmented bacterium, designated strain JXAS1, was isolated from a freshwater sample collected from Poyang Lake in China. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the isolate belonged to the genus , being closest to DSM 6368 (98.61 %). The genome size of strain JXAS1 was 4.66 Mb with DNA G+C content 35.7 mol%. The average nucleotide identity and DNA-DNA hybridization values between strain JXAS1 and its closest relatives were below the threshold values of 95 and 70 %, respectively. The strain contained menaquinone 6 (MK-6) as the predominant menaquinone and the major polar lipids were phosphatidylethanolamine, one unidentified glycolipid, and one unidentified polar lipid. The major fatty acids (>5 %) were iso-C, summed feature 3 (C 7 and/or C 6), C, iso-C 3OH, iso-C 3OH, and summed feature 9 (iso-C 9 and/or 10-methyl C). Based on phylogenetic, genotypic, and phenotypic evidence, the isolated strain represents a new species in the genus , and the name is proposed. The type strain is JXAS1 (=GDMCC 1.1378=KCTC 62719).
一株革兰氏阴性、需氧、不产芽孢、非运动、杆状、呈黄色的细菌，被命名为 JXAS1 菌株，从中国鄱阳湖的一个淡水样本中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，该分离物属于 属，与 DSM 6368 最为接近（98.61%）。JXAS1 菌株的基因组大小为 4.66 Mb，DNA G+C 含量为 35.7 mol%。JXAS1 菌株与最接近的亲缘菌株的平均核苷酸同一性和 DNA-DNA 杂交值均低于 95%和 70%的阈值。该菌株含有menaquinone 6 (MK-6)作为主要的menaquinone，主要的极性脂为磷脂酰乙醇胺、一种未鉴定的糖脂和一种未鉴定的极性脂。主要脂肪酸（>5%）为 iso-C、summed feature 3 (C 7 和/或 C 6)、C、iso-C 3OH、iso-C 3OH 和 summed feature 9 (iso-C 9 和/或 10-methyl C)。基于系统发育、基因型和表型证据，该分离株代表了 属中的一个新种，建议命名为 。其模式菌株为 JXAS1（=GDMCC 1.1378=KCTC 62719）。
